Methods and Protocols

  • Victoria A. Lawson

Part of the Methods in Molecular Biology book series (MIMB, volume 1658)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Methods for Biochemical and Biophysical Analysis of Prion Disease

    1. Front Matter
      Pages 1-1
    2. Natallia Makarava, Regina Savtchenko, Ilia V. Baskakov
      Pages 3-22
    3. Laura J. Ellett, Vanessa A. Johanssen
      Pages 27-34
    4. Gerald S. Baron, Gregory J. Raymond
      Pages 51-66
    5. Shayne A. Bellingham, Andrew F. Hill
      Pages 67-80
  3. Methods for Cellular Analysis of Prion Disease

    1. Front Matter
      Pages 81-81
    2. Zaira E. Arellano-Anaya, Alvina Huor, Pascal Leblanc, Olivier Andréoletti, Didier Vilette
      Pages 95-104
    3. Laura J. Vella, Bradley Coleman, Andrew F. Hill
      Pages 105-118
    4. Giuseppe D. Ciccotosto, Metta Jana, Roberto Cappai
      Pages 147-165
  4. Methods for Cell Free Propagation of Prions

    1. Front Matter
      Pages 167-167
    2. Natallia Makarava, Regina Savtchenko, Ilia V. Baskakov
      Pages 169-183
    3. Christina D. Orrù, Bradley R. Groveman, Andrew G. Hughson, Matteo Manca, Lynne D. Raymond, Gregory J. Raymond et al.
      Pages 185-203
    4. Natalia Fernández-Borges, Hasier Eraña, Saioa R. Elezgarai, Chafik Harrathi, Vanesa Venegas, Joaquín Castilla
      Pages 205-216
  5. In Vivo Investigation of Prion Disease

    1. Front Matter
      Pages 217-217
    2. Victoria A. Lawson, Carolin Tumpach, Cathryn L. Haigh, Simon C. Drew
      Pages 253-262
    3. Aileen Boyle, Kris Hogan, Jean C. Manson, Abigail B. Diack
      Pages 263-283
    4. Laura J. Ellett, Victoria A. Lawson
      Pages 285-292
  6. Methods for the Detection and Treatment of Prion Diseases

    1. Front Matter
      Pages 293-293
    2. Katsuya Satoh, Ryuichiro Atarashi, Noriyuki Nishida
      Pages 305-310
    3. Jonathan D. F. Wadsworth, Gary Adamson, Susan Joiner, Lara Brock, Caroline Powell, Jacqueline M. Linehan et al.
      Pages 311-346
    4. G. M. Klug, V. Lewis, S. J. Collins
      Pages 347-354
  7. Methods for Investigation of Prion-Like Disease

    1. Front Matter
      Pages 355-355
  8. Back Matter
    Pages 369-371

About this book


This volume details protocols on prion disease from multiple disciplines and highlights the contribution each discipline has made to the understanding of prion disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and practical, Prions: Methods and Protocols aims to ensure successful results in the further study of this vital field.


prion disease cellular analysis cell free propagation protein misfolding neurodegenerative diseases intercellular prion-like conversion

Editors and affiliations

  • Victoria A. Lawson
    • 1
  1. 1.Department of PathologyThe University of MelbourneParkvilleAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7242-5
  • Online ISBN 978-1-4939-7244-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site