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Innate Antiviral Immunity

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Karen Mossman

    1. Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada

    View editor publications

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  • Includes cutting-edge methods and protocols for the study of innate antiviral immunity
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1656)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The Application of Humanized Mouse Models for the Study of Human Exclusive Viruses

    • Fatemeh Vahedi, Elizabeth C. Giles, Ali A. Ashkar
    Pages 1-56

  3. Zebrafish as a Model for the Study of Host-Virus Interactions

    • Peng Fei Zou, Pin Nie
    Pages 57-78

  4. Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes

    • Tianyun Long, Rui Lu
    Pages 79-88

  5. Extraction and qPCR-Based Detection of miRNAs from Cultured PBMCs of Bubaline Origin

    • Chandra S. Mukhopadhyay, Ramneek Verma, Jasdeep Singh
    Pages 89-102

  6. Visualizing Virus-Derived dsRNA Using Antibody-Independent and -Dependent Methods

    • Sarah J. Poynter, Stephanie J. DeWitte-Orr
    Pages 103-118

  7. RNA PAMPs as Molecular Tools for Evaluating RIG-I Function in Innate Immunity

    • Renee C. Ireton, Courtney Wilkins, Michael Gale Jr.
    Pages 119-129

  8. Methods to Visualize MAVS Subcellular Localization

    • Christine Vazquez, Dia C. Beachboard, Stacy M. Horner
    Pages 131-142

  9. Purification of Cyclic GMP-AMP from Viruses and Measurement of Its Activity in Cell Culture

    • Alice Mayer, Jonathan Maelfait, Anne Bridgeman, Jan Rehwinkel
    Pages 143-152Open Access

  10. cGAMP Quantification in Virus-Infected Human Monocyte-Derived Cells by HPLC-Coupled Tandem Mass Spectrometry

    • Jennifer Paijo, Volkhard Kaever, Ulrich Kalinke
    Pages 153-166

  11. Methods of Assessing STING Activation and Trafficking

    • Vladislav Pokatayev, Nan Yan
    Pages 167-174

  12. Genome-Wide CRISPR/Cas9 Screening for High-Throughput Functional Genomics in Human Cells

    • Shiyou Zhu, Yuexin Zhou, Wensheng Wei
    Pages 175-181

  13. High-Throughput Screening for Identification of Novel Innate Immune Activators

    • Bryan J. Gall, Victor R. DeFilippis
    Pages 183-193

  14. Chromosome Conformation Capture for Research on Innate Antiviral Immunity

    • Yoon Jung Kim, Tae Hoon Kim
    Pages 195-208

  15. Discovery of Variants Underlying Host Susceptibility to Virus Infection Using Whole-Exome Sequencing

    • Gabriel A. Leiva-Torres, Nestor Nebesio, Silvia M. Vidal
    Pages 209-227

  16. Isolation, Purification, and Culture of Primary Murine Sensory Neurons

    • Sarah Katzenell, Jorge R. Cabrera, Brian J. North, David A. Leib
    Pages 229-251

  17. Isolation of Group 2 Innate Lymphoid Cells from Mouse Lungs

    • Claudia U. Duerr, Jörg H. Fritz
    Pages 253-261

  18. Epidemiological Methods

    • Biao Wang, Mark Loeb
    Pages 263-271

  19. Erratum to: Purification of Cyclic GMP-AMP from Viruses and Measurement of Its Activity in Cell Culture

    • Alice Mayer, Jonathan Maelfait, Anne Bridgeman, Jan Rehwinkel
    Pages E1-E1

  20. Correction to: Isolation, Purification, and Culture of Primary Murine Sensory Neurons

    • Sarah Katzenell, Jorge R. Cabrera, Brian J. North, David A. Leib
    Pages E3-E3
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Keywords

About this book

This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and virus-derived dsRNA from a variety of cells, tissues, and organisms, as well as to interrogating the cytosolic RNA and DNA sensing pathways, including using RNA PAMPs as molecular tools, purification of cGAMP from virus particles and infected cells, and mechanisms to visualize the subcellular localization and activation of the adaptor proteins MAVS and STING. Cutting-edge methods, including high-throughput and genome-wide CRISPR/Cas9 screens, chromosome conformation capture, and whole-exome sequencing, are described to identify novel mediators, pathways, and variants underlying host susceptibility. Given the importance of studying these pathways and players under physiologic conditions, methods describing the isolation of primary mouse sensory neurons and group 2 innate lymphoid cells are also provided. Finally, this collection comes full circle back to the whole organism level and concludes with epidemiological methods to investigate virus-host interactions and the induction of innate immunity. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Innate Antiviral Immunity: Methods and Protocols spans a diverse array of approaches to study and elucidate the intricacies of this vital area of study.


The chapter 'Morphological Separation ofClustered Nuclei in Histological Images' is published open access under a CC BY 4.0 license at link.springer.com. 

Editors and Affiliations

  • Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada

    Karen Mossman

Bibliographic Information

  • Book Title: Innate Antiviral Immunity

  • Book Subtitle: Methods and Protocols

  • Editors: Karen Mossman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7237-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7236-4Published: 14 August 2017

  • Softcover ISBN: 978-1-4939-8426-8Published: 03 August 2018

  • eBook ISBN: 978-1-4939-7237-1Published: 13 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 275

  • Number of Illustrations: 11 b/w illustrations, 30 illustrations in colour

  • Topics: Immunology

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