Microbial Steroids

Methods and Protocols

  • José-Luis Barredo
  • Ignacio Herráiz

Part of the Methods in Molecular Biology book series (MIMB, volume 1645)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Marina V. Donova
    Pages 1-13
  3. Victoria Y. Shtratnikova, Mikhail I. Schelkunov, Marina V. Donova
    Pages 29-44
  4. Alberto Sola-Landa, Antonio Rodríguez-García, Carlos Barreiro, Rosario Pérez-Redondo
    Pages 45-58
  5. Antonio Rodríguez-García, Alberto Sola-Landa, Carlos Barreiro
    Pages 59-72
  6. Carlos Barreiro, Alejandro Morales, Inés Vázquez-Iglesias, Alberto Sola-Landa
    Pages 73-92
  7. María-Angélica Mondaca, Maricel Vidal, Soledad Chamorro, Gladys Vidal
    Pages 143-150
  8. Soledad Chamorro, Claudia Xavier, Víctor Hernández, José Becerra, Gladys Vidal
    Pages 151-158
  9. Rodrigo A. Mancilla, Roberto Pavez-Díaz, Alejandro Amoroso
    Pages 159-165
  10. Marco P. C. Marques, Pedro Fernandes
    Pages 167-176
  11. Kjell D. Josefsen, Anna Nordborg, Håvard Sletta
    Pages 177-197
  12. Sonia Martínez-Cámara, Esther Bahíllo, José-Luis Barredo, Marta Rodríguez-Sáiz
    Pages 199-210
  13. Julia García-Fernández, Igor Martínez, Lorena Fernández-Cabezón, Carmen Felpeto-Santero, José-Luis García, Beatriz Galán
    Pages 211-225
  14. Sabrina Hoebenreich, Martin Spinck, Nathalie Nett
    Pages 239-257
  15. Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, Marina V. Donova
    Pages 259-269
  16. Lidia Ortega-de los Ríos, José M. Luengo, José M. Fernández-Cañón
    Pages 271-287
  17. Hui Li, Jin Sun, Zhenghong Xu
    Pages 289-295
  18. Ana Luisa Ribeiro, Mercedes Sánchez, Aurelio Hidalgo, José Berenguer
    Pages 297-312
  19. Carla C. C. R. de Carvalho, Pedro Fernandes
    Pages 313-320
  20. Fabian B. Thygs, Juliane Merz
    Pages 321-345
  21. Govinda Guevara, Julián Perera, Juana-María Navarro-Llorens
    Pages 347-360
  22. Back Matter
    Pages 369-373

About this book


This volume provides practical experimental laboratory protocols for a wide range of steroid bioconversions. The chapters in this book cover topics such as bioconversions and chemical synthesis pathways; strain characterization; bioconversion from sterols to androstenedione and androstadienedione; steroid hydroxylations; biocatalysis; and downstream processes to purify steroid intermediates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Microbial Steroids: Methods and Protocols is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology.


Terpenoid lipids Steroid molecules Hormones Bioconversions Chemical sythesis

Editors and affiliations

  • José-Luis Barredo
    • 1
  • Ignacio Herráiz
    • 2
  1. 1.Department of Biotechnology, Crystal Pharma, A Division of Albany Molecular Research Inc. (AMRI)Parque Tecnológico de LeónLeónSpain
  2. 2.Research and Development Department, Gadea Pharmaceutical Group, A Division of Albany Molecular Research Inc. (AMRI)Parque Tecnológico de BoecilloBoecilloSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7182-4
  • Online ISBN 978-1-4939-7183-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site