Plant Argonaute Proteins

Methods and Protocols

  • Alberto Carbonell

Part of the Methods in Molecular Biology book series (MIMB, volume 1640)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Károly Fátyol, József Burgyán
    Pages 73-91
  3. Cécile Lecampion, Elodie Lanet, Christophe Robaglia
    Pages 137-143
  4. Marina Martinez-Garcia, Mónica Pradillo
    Pages 145-158
  5. Meizhen Wang, Yuxing Deng, Fenjuan Shao, Miaomiao Liu, Yongqi Pang, Caili Li et al.
    Pages 173-189
  6. Zhiqiang Xian, Fang Yan, Zhengguo Li
    Pages 191-197
  7. Ariel H. Tomassi, Delfina Gagliardi, Damian A. Cambiagno, Pablo A. Manavella
    Pages 199-210
  8. Kai Tang, Cheng-Guo Duan, Huiming Zhang, Jian-Kang Zhu
    Pages 219-225
  9. Andrzej Zielezinski, Wojciech M. Karlowski
    Pages 241-256
  10. Joshua T. Trujillo, Rebecca A. Mosher
    Pages 257-266
  11. Ravi K. Singh, Shree P. Pandey
    Pages 267-294
  12. Back Matter
    Pages 295-297

About this book


This volume provides the reader with step–by–step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO–small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non–radioactive protocols for small RNA detection.  Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function.


ARGONAUTEs RIP-seq target RNA mass spectrometry Affinity Purification

Editors and affiliations

  • Alberto Carbonell
    • 1
  1. 1.Instituto de Biología Molecular y Celular de Plantas(Consejo Superior de Investigaciones Científicas–Universidad Politécnica de Valencia)ValenciaSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7164-0
  • Online ISBN 978-1-4939-7165-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site