The Bacterial Nucleoid

Methods and Protocols

  • Olivier Espéli

Part of the Methods in Molecular Biology book series (MIMB, volume 1624)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Molecular Genetic Methods to Study Bacterial Nucleoid

  3. Study of Bacterial Nucleoid with Whole Genome Analysis Method

    1. Front Matter
      Pages 51-51
    2. Mickaël Poidevin, Elisa Galli, Yoshiharu Yamaichi, François-Xavier Barre
      Pages 53-60
    3. Roxanne E. Diaz, Aurore Sanchez, Véronique Anton Le Berre, Jean-Yves Bouet
      Pages 61-73
    4. Martial Marbouty, Romain Koszul
      Pages 75-84
    5. Parul Singh, Aswin Sai Narain Seshasayee
      Pages 85-97
  4. Molecular Biology Methods to Study Nucleoid Structuring Factors

    1. Front Matter
      Pages 99-99
    2. Sanja Mehandziska, Alexander M. Petrescu, Georgi Muskhelishvili
      Pages 101-116
    3. Ramon A. van der Valk, Niels Laurens, Remus T. Dame
      Pages 127-143
    4. Zoya M. Petrushenko, Valentin V. Rybenkov
      Pages 145-159
    5. Rupa Sarkar, Valentin V. Rybenkov
      Pages 161-171
    6. Ricksen S. Winardhi, Jie Yan
      Pages 173-191
    7. Damien Larivière, Rodrigo Galindo-Murillo, Eric Fourmentin, Samuel Hornus, Bruno Lévy, Julie Papillon et al.
      Pages 193-210
  5. Imaging the Bacterial Nucleoid

    1. Front Matter
      Pages 211-211
    2. Bryan J. Visser, Mohan C. Joshi, David Bates
      Pages 213-226
    3. Gaëlle Demarre, Victoria Prudent, Olivier Espéli
      Pages 227-236
    4. Roy de Leeuw, Peter Brazda, M. Charl Moolman, J. W. J. Kerssemakers, Belen Solano, Nynke H. Dekker
      Pages 237-252
    5. Antoine Le Gall, Diego I. Cattoni, Marcelo Nollmann
      Pages 253-268
    6. Christoph Spahn, Mathilda Glaesmann, Yunfeng Gao, Yong Hwee Foo, Marko Lampe, Linda J. Kenney et al.
      Pages 269-289
  6. Biophysics of the Bacterial Nucleoid

  7. Christoph Spahn, Mathilda Glaesmann, Yunfeng Gao, Yong Hwee Foo, Marko Lampe, Linda J. Kenney et al.
    Pages E1-E1
  8. Back Matter
    Pages 373-374

About this book


This volume presents state-of-the-art protocols for key experiments that have revolutionized our understanding of the bacterial nucleoid. This book is divided into five parts: Part I introduces molecular genetic methods to study bacterial nucleoids; Part II highlights the study of bacterial nucleoid with whole genome analysis method; Part III discusses molecular biology methods to study nucleoid structuring factors; Part IV looks at imaging bacterial nucleoid; and Part V explores biophysics of the bacterial nucleoid. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, The Bacterial Nucleoid: Methods and Protocols is a valuable resource that provides a wealth of new information about this chromosome.


eukaryotic prokaryotic deep-sequencing DNA replication chromosome segregation

Editors and affiliations

  • Olivier Espéli
    • 1
  1. 1.UMR CNRS 7241, INSERM U1050CIRB, Collège de FranceParisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7097-1
  • Online ISBN 978-1-4939-7098-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site