Serum/Plasma Proteomics

Methods and Protocols

  • David W. Greening
  • Richard J. Simpson

Part of the Methods in Molecular Biology book series (MIMB, volume 1619)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Updated Blood Processing and Handling Strategies

    1. Front Matter
      Pages 1-1
    2. Viviana Greco, Cristian Piras, Luisa Pieroni, Andrea Urbani
      Pages 3-21
    3. Rosemary L. Sparrow, Richard J. Simpson, David W. Greening
      Pages 23-30
    4. David W. Greening, Richard J. Simpson, Rosemary L. Sparrow
      Pages 31-42
  3. Updated Fractionation Strategies for In-depth Blood Proteome Analysis

    1. Front Matter
      Pages 43-43
    2. Elin Birgersson, Jochen M. Schwenk, Burcu Ayoglu
      Pages 45-54
    3. John P. O’Grady, Kevin W. Meyer, Derrick N. Poe
      Pages 55-61
    4. David W. Greening, Richard J. Simpson
      Pages 63-79
    5. Lynn A. Beer, Bonnie Ky, Kurt T. Barnhart, David W. Speicher
      Pages 81-101
    6. Dong Huey Cheon, Eun Gyeong Yang, Cheolju Lee, Ji Eun Lee
      Pages 103-117
    7. Qichen Cao, Qing Zhao, Xiaohong Qian, Wantao Ying
      Pages 127-137
  4. Updated Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids

    1. Front Matter
      Pages 139-139
    2. Montserrat Baldan-Martin, Fernando de la Cuesta, Gloria Alvarez-Llamas, Gema Ruiz-Hurtado, Luis M. Ruilope, Maria G. Barderas
      Pages 141-149
    3. Tania Martins-Marques, Sandra I. Anjo, Teresa Ribeiro-Rodrigues, Bruno Manadas, Henrique Girao
      Pages 151-172
    4. Ana Sofia Carvalho, Manuel S. Rodriguez, Rune Matthiesen
      Pages 173-181
    5. Nicolas Christinat, Delphine Morin-Rivron, Mojgan Masoodi
      Pages 183-191
    6. Susanne Sales, Oskar Knittelfelder, Andrej Shevchenko
      Pages 203-212
    7. Antonius Koller, Purvi Patel, Jenny Kim Kim, Emily I. Chen
      Pages 213-225
  5. Antibody-based Discovery and Targeted Proteomics

    1. Front Matter
      Pages 227-227
    2. Cecilia Hellström, Tea Dodig-Crnković, Mun-Gwan Hong, Jochen M. Schwenk, Peter Nilsson, Ronald Sjöberg
      Pages 229-238
    3. Véronique Laforte, Pik-Shan Lo, Huiyan Li, David Juncker
      Pages 239-261
    4. Larissa Belov, Susannah Hallal, Kieran Matic, Jerry Zhou, Sandra Wissmueller, Nuzhat Ahmed et al.
      Pages 263-301
    5. Shabarni Gupta, K. P. Manubhai, Shuvolina Mukherjee, Sanjeeva Srivastava
      Pages 303-315
  6. Developments in Discovery and Targeted Proteomics

    1. Front Matter
      Pages 317-317
    2. Pengyuan Liu, Lynn A. Beer, Bonnie Ky, Kurt T. Barnhart, David W. Speicher
      Pages 319-337
    3. Lynn A. Beer, Pengyuan Liu, Bonnie Ky, Kurt T. Barnhart, David W. Speicher
      Pages 339-352
    4. Julie A. Reisz, Katelyn M. Chessler, Monika Dzieciatkowska, Angelo D’Alessandro, Kirk C. Hansen
      Pages 353-371
    5. Christoph Krisp, Mark P. Molloy
      Pages 373-383
    6. Garikapati Vannuruswamy, Arvind M. Korwar, Mashanipalya G. Jagadeeshaprasad, Mahesh J. Kulkarni
      Pages 403-416
  7. Developments in Biomarker Discovery

    1. Front Matter
      Pages 431-431
    2. Loïc Dayon, Antonio Núñez Galindo, Ornella Cominetti, John Corthésy, Martin Kussmann
      Pages 433-449
    3. Santosh D. Bhosale, Robert Moulder, Petri Kouvonen, Riitta Lahesmaa, David R. Goodlett
      Pages 451-466
    4. Peiyuan Yin, Guowang Xu
      Pages 467-475
    5. Edmond J. Breen
      Pages 495-537
  8. Back Matter
    Pages 539-551

About this book


Over the past decade, there has been an increase in powerful proteomics technologies that allow greater fundamental insights into the blood proteome. Further developments in informatic analyses, software developments, and computational tools are providing insights into large data sets, open-source data along with large-scale application of bioinformatics. Serum/Plasma Proteomics: Methods and Protocols, Second Edition is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, covering fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays,  proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, data management, statistical design, and bioinformatic challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Serum/Plasma Proteomics: Methods and Protocols, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesicles/exosomes, and related biofluids.


Human blood serum components blood proteomics post-translational modifications blood proteome analysis blood cell components

Editors and affiliations

  • David W. Greening
    • 1
  • Richard J. Simpson
    • 2
  1. 1.Department of Biochemistry and GeneticsLa Trobe Institute for Molecular Science, La Trobe UniversityMelbourneAustralia
  2. 2.Department of Biochemistry and GeneticsLa Trobe Institute for Molecular Science, La Trobe UniversityMelbourneAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7056-8
  • Online ISBN 978-1-4939-7057-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site