3D Cell Culture

Methods and Protocols

  • Zuzana Koledova

Part of the Methods in Molecular Biology book series (MIMB, volume 1612)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Zuzana Koledova
    Pages 1-11
  3. Hydrogels and Scaffolds for 3D Cell Culture

    1. Front Matter
      Pages 13-13
    2. Bryan N. Brown, Michael J. Buckenmeyer, Travis A. Prest
      Pages 15-27
    3. Laura J. Bray, Marcus Binner, Uwe Freudenberg, Carsten Werner
      Pages 39-63
    4. Massimiliano Caiazzo, Yoji Tabata, Matthias P. Lutolf
      Pages 65-78
    5. Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, Maria-Pau Ginebra
      Pages 79-94
  4. 3D Organoid and Organotypic Cultures

    1. Front Matter
      Pages 95-95
    2. Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, Christina H. Scheel
      Pages 125-137
    3. Daniel H. Miller, Ethan S. Sokol, Piyush B. Gupta
      Pages 139-147
    4. Anas Rabata, Ales Hampl, Zuzana Koledova
      Pages 149-165
    5. Theresa Eder, Iris E. Eder
      Pages 167-175
    6. Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni et al.
      Pages 177-190
    7. Christian Reuter, Heike Walles, Florian Groeber
      Pages 191-198
    8. Romana E. Ranftl, Fernando Calvo
      Pages 199-212 Open Access
  5. Micropatterning

    1. Front Matter
      Pages 223-223
    2. J. Lowry Curley, Michael J. Moore
      Pages 225-237
    3. Michele M. Nava, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, Manuela T. Raimondi
      Pages 253-266
  6. Microfluidic Approaches for 3D Cell Culture

    1. Front Matter
      Pages 267-267
    2. Fabien Bertillot, Youmna Attieh, Morgan Delarue, Basile G. Gurchenkov, Stephanie Descroix, Danijela Matic Vignjevic et al.
      Pages 269-279
    3. Paola Occhetta, Roberta Visone, Marco Rasponi
      Pages 303-323
    4. Xiaolin Wang, Duc T. T. Phan, Steven C. George, Christopher C. W. Hughes, Abraham P. Lee
      Pages 325-344
    5. Kambez H. Benam, Marc Mazur, Youngjae Choe, Thomas C. Ferrante, Richard Novak, Donald E. Ingber
      Pages 345-365
  7. Bioprinting

    1. Front Matter
      Pages 367-367
    2. Cristina Colosi, Marco Costantini, Andrea Barbetta, Mariella Dentini
      Pages 369-380
    3. Guifang Gao, Karen Hubbell, Arndt F. Schilling, Guohao Dai, Xiaofeng Cui
      Pages 391-398
  8. Imaging and Image Analysis of 3D Cell Cultures

    1. Front Matter
      Pages 399-399
    2. Loredana Spoerri, Kimberley A. Beaumont, Andrea Anfosso, Nikolas K. Haass
      Pages 401-416
    3. Josef Jaros, Michal Petrov, Marketa Tesarova, Ales Hampl
      Pages 417-431
    4. Malin Åkerfelt, Mervi Toriseva, Matthias Nees
      Pages 433-445
  9. Massimiliano Caiazzo, Yoji Tabata, Matthias P. Lutolf
    Pages E1-E1
  10. Back Matter
    Pages 447-452

About this book


This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures.

Chapter 15 of this book is available open access under a CC BY 4.0 license.


Hydrogels 3D Organoids Scaffolds Organotypic cultures Micropatterning Microfluidics Bioprinting Three-dimensional cell culture

Editors and affiliations

  • Zuzana Koledova
    • 1
  1. 1.Department of Histology and EmbryologyMasaryk UniversityBrnoCzech Republic

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7019-3
  • Online ISBN 978-1-4939-7021-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site