Book Volume 1607 2017

Protein Crystallography

Methods and Protocols

Editors:

ISBN: 978-1-4939-6998-2 (Print) 978-1-4939-7000-1 (Online)

Table of contents (27 protocols)

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  1. Front Matter

    Pages i-xiii

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    Protocol

    Pages 1-15

    Expression and Purification of Recombinant Proteins in Escherichia coli with a His6 or Dual His6-MBP Tag

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    Protocol

    Pages 17-50

    Protein Crystallization

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    Protocol

    Pages 51-76

    Advanced Methods of Protein Crystallization

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    Protocol

    Pages 77-115

    The “Sticky Patch” Model of Crystallization and Modification of Proteins for Enhanced Crystallizability

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    Protocol

    Pages 117-141

    Crystallization of Membrane Proteins: An Overview

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    Protocol

    Pages 143-164

    Locating and Visualizing Crystals for X-Ray Diffraction Experiments

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    Protocol

    Pages 165-184

    Collection of X-Ray Diffraction Data from Macromolecular Crystals

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    Protocol

    Pages 185-217

    Identifying and Overcoming Crystal Pathologies: Disorder and Twinning

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    Protocol

    Pages 219-238

    Applications of X-Ray Micro-Beam for Data Collection

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    Protocol

    Pages 239-272

    Serial Synchrotron X-Ray Crystallography (SSX)

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    Protocol

    Pages 273-294

    Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources

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    Protocol

    Pages 295-324

    Structure Determination Using X-Ray Free-Electron Laser Pulses

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    Protocol

    Pages 325-347

    Processing of XFEL Data

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    Protocol

    Pages 349-356

    Many Ways to Derivatize Macromolecules and Their Crystals for Phasing

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    Protocol

    Pages 357-376

    Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX

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    Protocol

    Pages 377-399

    Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis

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    Protocol

    Pages 401-420

    Long-Wavelength X-Ray Diffraction and Its Applications in Macromolecular Crystallography

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    Protocol

    Pages 421-453

    Acknowledging Errors: Advanced Molecular Replacement with Phaser

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    Protocol

    Pages 455-466

    Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems

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    Protocol

    Pages 467-489

    Radiation Damage in Macromolecular Crystallography

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