Heterologous Protein Production in CHO Cells

Methods and Protocols

  • Paula Meleady

Part of the Methods in Molecular Biology book series (MIMB, volume 1603)

Table of contents

  1. Front Matter
    Pages i-x
  2. Qiong Wang, Bojiao Yin, Cheng-Yu Chung, Michael J. Betenbaugh
    Pages 25-44
  3. Yashas Rajendra, Sowmya Balasubramanian, David L. Hacker
    Pages 45-55
  4. Eric Baek, Soo Min Noh, Gyun Min Lee
    Pages 71-85
  5. Alan Costello, Nga Lao, Martin Clynes, Niall Barron
    Pages 87-100
  6. Lise Marie Grav, Karen Julie la Cour Karottki, Jae Seong Lee, Helene Faustrup Kildegaard
    Pages 101-118
  7. Hussain Dahodwala, Susan T. Sharfstein
    Pages 153-168
  8. Craig Monger, Krishna Motheramgari, John McSharry, Niall Barron, Colin Clarke
    Pages 169-186
  9. Michael Henry, Orla Coleman, Prashant, Martin Clynes, Paula Meleady
    Pages 195-208
  10. Yuzhou Fan, Helene Faustrup Kildegaard, Mikael Rørdam Andersen
    Pages 209-226
  11. Sara Carillo, Stefan Mittermayr, Amy Farrell, Simone Albrecht, Jonathan Bones
    Pages 227-241
  12. Catherine E. M. Hogwood, Lesley M. Chiverton, C. Mark Smales
    Pages 243-250
  13. Back Matter
    Pages 251-252

About this book

Introduction

This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.

Keywords

CRISPR Cas9 bioinformatic recombinant protein CHO-K1

Editors and affiliations

  • Paula Meleady
    • 1
  1. 1.National Institute for Cellular BiotechnologyDublin City UniversityDublinIreland

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6972-2
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6971-5
  • Online ISBN 978-1-4939-6972-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book