Reverse Genetics of RNA Viruses

Methods and Protocols

  • Daniel R. Perez

Part of the Methods in Molecular Biology book series (MIMB, volume 1602)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Johnasha D. Stuart, Matthew B. Phillips, Karl W. Boehme
    Pages 1-10
  3. Mingyuan Han, Hanzhong Ke, Yijun Du, Qingzhan Zhang, Dongwan Yoo
    Pages 29-46
  4. Chao Shan, Xuping Xie, Pei-Yong Shi
    Pages 47-58
  5. Erica Bickerton, Sarah M. Keep, Paul Britton
    Pages 83-102
  6. Shringkhala Bajimaya, Tsuyoshi Hayashi, Toru Takimoto
    Pages 103-110
  7. Christopher C. Stobart, Anne L. Hotard, Jia Meng, Martin L. Moore
    Pages 111-124
  8. B. G. van den Hoogen, R. A. M. Fouchier
    Pages 125-139
  9. Stivalis Cardenas-Garcia, Claudio L. Afonso
    Pages 141-158
  10. Thomas Hoenen, Heinz Feldmann
    Pages 159-170
  11. Luis Martínez-Sobrido, Slobodan Paessler, Juan Carlos de la Torre
    Pages 185-204
  12. Aitor Nogales, Daniel R. Perez, Jefferson Santos, Courtney Finch, Luis Martínez-Sobrido
    Pages 205-238
  13. Daniela Toro-Ascuy, Marcelo Cortez-San Martín
    Pages 239-250
  14. Daniel R. Perez, Matthew Angel, Ana Silvia Gonzalez-Reiche, Jefferson Santos, Adebimpe Obadan, Luis Martinez-Sobrido
    Pages 251-273
  15. Back Matter
    Pages 275-282

About this book


This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.


reverse genetics RNA virus gene sequences de novo reconstitution cDNA copy

Editors and affiliations

  • Daniel R. Perez
    • 1
  1. 1.Department of Population Health, Poultry Diagnostic and Research Center, College of Veterinary MedicineUniversity of GeorgiaAthensUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6962-3
  • Online ISBN 978-1-4939-6964-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site