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Synthetic Protein Switches

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols for the study of synthetic protein switches in biotechnology
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1596)

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Table of contents (20 protocols)

  1. Optogenetic Switches

    1. Front Matter

      Pages 239-239
    2. Light-Regulated Protein Kinases Based on the CRY2-CIB1 System

      • Wignand W. D. Mühlhäuser, Maximilian Hörner, Wilfried Weber, Gerald Radziwill
      Pages 257-270
    3. Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels

      • Cristian Cosentino, Laura Alberio, Gerhard Thiel, Anna Moroni
      Pages 271-285
    4. Primer-Aided Truncation for the Creation of Hybrid Proteins

      • Robert Stabel, Birthe Stüven, Robert Ohlendorf, Andreas Möglich
      Pages 287-304
  2. Cellular Signaling Switches

    1. Front Matter

      Pages 305-305
    2. Engineering Small Molecule Responsive Split Protein Kinases

      • Javier Castillo-Montoya, Indraneel Ghosh
      Pages 307-319
    3. Directed Evolution Methods to Rewire Signaling Networks

      • Raphaël B. Di Roberto, Benjamin M. Scott, Sergio G. Peisajovich
      Pages 321-337
  3. Back Matter

    Pages 339-341

About this book

This detailed book provides state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, β-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Synthetic Protein Switches: Methods and Protocols serves as a useful reference for life scientists from diverse research fields that range from traditional, discovery-centered disciplines, like cancer research, to newly emerging disciplines, such as synthetic biology.

Editors and Affiliations

  • Fachbereich Biologie, Technische Universität Darmstadt, Darmstadt, Germany

    Viktor Stein

Bibliographic Information

  • Book Title: Synthetic Protein Switches

  • Book Subtitle: Methods and Protocols

  • Editors: Viktor Stein

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6940-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6938-8Published: 15 March 2017

  • Softcover ISBN: 978-1-4939-8345-2Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6940-1Published: 13 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 341

  • Number of Illustrations: 28 b/w illustrations, 31 illustrations in colour

  • Topics: Biotechnology

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 129.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access