The Bacterial Flagellum

Methods and Protocols

  • Tohru Minamino
  • Keiichi Namba

Part of the Methods in Molecular Biology book series (MIMB, volume 1593)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Flagellar Type III Protein Export, Assembly and Gene Regulation in Salmonella enterica

    1. Front Matter
      Pages 1-1
    2. Tohru Minamino, Miki Kinoshita, Keiichi Namba
      Pages 3-16
    3. Lewis D. B. Evans, Paul M. Bergen, Owain J. Bryant, Gillian M. Fraser
      Pages 17-35
    4. Fabienne F. V. Chevance, Kelly T. Hughes
      Pages 47-71
    5. Santosh Koirala, Christopher V. Rao
      Pages 73-83
  3. Structure of the Bacterial Flagellum

  4. Dynamics of the Bacterial Flagellar Motor

    1. Front Matter
      Pages 161-161
    2. Yuichi Inoue
      Pages 163-174
    3. Taishi Kasai, Yoshiyuki Sowa
      Pages 185-192
    4. Tsai-Shun Lin, Yi-Ren Sun, Chien-Jung Lo
      Pages 193-201
    5. Yusuke V. Morimoto, Tohru Minamino
      Pages 203-213
  5. Structural Diversity of the Bacterial Flagellar Motors Derived from Different Bacterial Species

    1. Front Matter
      Pages 227-227
    2. Shiwei Zhu, Zhuan Qin, Juyu Wang, Dustin R. Morado, Jun Liu
      Pages 229-242
    3. Shuichi Nakamura, Md. Shafiqul Islam
      Pages 243-251
    4. Yasuhiro Onoue, Michio Homma
      Pages 253-258
    5. Ikuro Kawagishi, So-ichiro Nishiyama
      Pages 259-271
    6. Javier de la Mora, Laura Camarena, Georges Dreyfus
      Pages 273-283
    7. Susanne Brenzinger, Kai M. Thormann
      Pages 285-295
  6. Back Matter
    Pages 321-326

About this book


This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species.


MotAB proton channel flagellar gene expression supramolecular motility machinery signal transduction Salmonella enterica

Editors and affiliations

  • Tohru Minamino
    • 1
  • Keiichi Namba
    • 2
  1. 1.Graduate School of Frontier BiosciencesOsaka UniversitySuitaJapan
  2. 2.Quantitative Biology Center RIKENSuitaJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6926-5
  • Online ISBN 978-1-4939-6927-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site