Protein-Carbohydrate Interactions

Methods and Protocols

  • D. Wade Abbott
  • Alicia Lammerts van Bueren

Part of the Methods in Molecular Biology book series (MIMB, volume 1588)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Analysis of Carbohydrate-Active Enzyme Activity

    1. Front Matter
      Pages 1-1
    2. Gregory Arnal, Mohamed A. Attia, Jathavan Asohan, Harry Brumer
      Pages 3-14
    3. Darryl R. Jones, Richard McLean, D. Wade Abbott
      Pages 37-44
    4. Galina Mai-Gisondi, Emma R. Master
      Pages 45-57
    5. Maria Ngo, Michael D. L. Suits
      Pages 59-70
    6. Bjørge Westereng, Magnus Ø. Arntzen, Jane Wittrup Agger, Gustav Vaaje-Kolstad, Vincent G. H. Eijsink
      Pages 71-92
    7. Johanna Stern, Lior Artzi, Sarah Moraïs, Carlos M. G. A. Fontes, Edward A. Bayer
      Pages 93-116
  3. Analysis of Protein-Carbohydrate Interactions

    1. Front Matter
      Pages 117-117
    2. Darrell Cockburn, Casper Wilkens, Birte Svensson
      Pages 119-127
    3. Haiyang Wu, Cédric Y. Montanier, Claire Dumon
      Pages 129-141
    4. Julie M. Grondin, David N. Langelaan, Steven P. Smith
      Pages 143-156
    5. Daniel J. Cosgrove, Nathan K. Hepler, Edward R. Wagner, Daniel M. Durachko
      Pages 157-165
  4. Visualization of Carbohydrates and Protein-Carbohydrate Complexes

    1. Front Matter
      Pages 167-167
    2. Louise Badruna, Vincent Burlat, Cédric Y. Montanier
      Pages 181-197
    3. Richard McLean, G. Douglas Inglis, Steven C. Mosimann, Richard R. E. Uwiera, D. Wade Abbott
      Pages 199-208
    4. Mélissa Robb, Joanne K. Hobbs, Alisdair B. Boraston
      Pages 215-221
  5. CAZyme Structure, Discovery, and Prediction Methods

    1. Front Matter
      Pages 237-237
    2. Benoit J. Kunath, Andreas Bremges, Aaron Weimann, Alice C. McHardy, Phillip B. Pope
      Pages 255-277
    3. Robert J. Gruninger, Ian Reid, Robert J. Forster, Adrian Tsang, Tim A. McAllister
      Pages 279-298
  6. Galina Mai-Gisondi, Emma R. Master
    Pages E1-E3
  7. Back Matter
    Pages 309-311

About this book


This volume is a wide-ranging tool for studying protein-carbohydrate interactions that extend from traditional biochemical methods to state-of-the-art techniques. This book focuses on four different research themes: Part I describes methods for screening and quantifying CAZyme activity; Part II contains methods for investigating the interactions between proteins and carbohydrate ligands; Part III discusses methods for the visualization of carbohydrates and protein-carbohydrate complexes; and Part IV focuses on structural and “omic” approaches for studying systems of CAZymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein-Carbohydrate Interactions: Methods and Protocols is a valuable resource to the glycomics research community. In this continuously advancing field, the methods in this book highlight the biology of glycomics, thus driving biotechnological innovation and solutions for human health and sustainable resources within the emerging green community. 


Carbohydrate active enzymes CAZymes Enzyme discovery Enzyme function quantification Visualization

Editors and affiliations

  • D. Wade Abbott
    • 1
  • Alicia Lammerts van Bueren
    • 2
  1. 1.Agriculture and Agri-Food CanadaLethbridgeCanada
  2. 2.Biotechnology InstituteUniversity of GroningenGroningenThe Netherlands

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6898-5
  • Online ISBN 978-1-4939-6899-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site