Table of contents
About this book
This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and validation. This section is completed by a brief description of a bioinformatics platform that supports antibody engineering during research and development. Part II contains basic procedures about the selection and characterization of aptamer molecules, and Part III describes fundamental processes of MIP generation and application. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Synthetic Antibodies: Methods and Protocols is an ideal guide for scientists seeking to propel the vital study of antibody research.
Amino acid based antibodies Affinity reagents Aptamers Nucleotides Moleculary imprinted polymers Immunoglobulin Scaffold engineering
Editors and affiliations
- DOI https://doi.org/10.1007/978-1-4939-6857-2
- Copyright Information Springer Science+Business Media LLC 2017
- Publisher Name Humana Press, New York, NY
- eBook Packages Springer Protocols
- Print ISBN 978-1-4939-6855-8
- Online ISBN 978-1-4939-6857-2
- Series Print ISSN 1064-3745
- Series Online ISSN 1940-6029
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