Ethylene Signaling

Methods and Protocols

  • Brad M. Binder
  • G. Eric Schaller

Part of the Methods in Molecular Biology book series (MIMB, volume 1573)

Table of contents

  1. Front Matter
    Pages i-x
  2. Analysis of Ethylene Biosynthesis

  3. Analysis of the Ethylene Signaling Pathway

  4. Analysis of Ethylene Responses

  5. Back Matter
    Pages 269-272

About this book


This volume provides a collection of protocols aimed toward the study of ethylene signaling in plants. Ethylene Signaling: Methods and Protocols is divided into three sections: ethylene biosynthesis, the signal transduction pathway, and the diverse ethylene responses of dicots and monocots. The chapters in section one discuss techniques for the measurement of activities related to the biosynthetic enzymes ACC synthase and ACC oxidase, the levels of ethylene synthesized by plants, and the treatment of plants with exogenous ethylene. Section two focuses on the analysis of the new membrane-associated proteins involved in the initial perception and transduction of the ethylene signal, such as ethylene receptors, CTR1, and EIN2. The third section covers assays applicable to dicots and monocots, including methods related to the roles of ethylene in germination, growth, abscission, abiotic stress, and defense. Section three also includes information on Arabidopsis mutants and the variety of chemical inhibitors that affect ethylene responses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and comprehensive, Ethylene Signaling: Methods and Protocols is a valuable resource for both experienced and beginner researchers with prior experience in the study of ethylene signaling and for those who are just entering this exciting research field.



hormone illuminating gas biosynthetic enzymes ACC synthase and ACC oxidase plants with exogenous ethylene ethylene receptors CTR1 EIN2 Arabidopsis

Editors and affiliations

  • Brad M. Binder
    • 1
  • G. Eric Schaller
    • 2
  1. 1.Department of Biochemistry & Cellular and Molecular BiologyUniversity of TennesseeKnoxvilleUSA
  2. 2.Department of Biological SciencesDartmouth CollegeHanoverUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6852-7
  • Online ISBN 978-1-4939-6854-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site