Thermogenic Fat

Methods and Protocols

  • Jun Wu

Part of the Methods in Molecular Biology book series (MIMB, volume 1566)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Isolation and Culture Methods

    1. Front Matter
      Pages 1-1
    2. Longhua Liu, Louise D. Zheng, Sarah R. Donnelly, Margo P. Emont, Jun Wu, Zhiyong Cheng
      Pages 9-16
    3. Umesh D. Wankhade, Sushil G. Rane
      Pages 17-24
    4. Jochen Steinbring, Antonia Graja, Anne-Marie Jank, Tim J. Schulz
      Pages 25-36
    5. Rohollah Babaei, Irem Bayindir-Buchhalter, Irina Meln, Alexandros Vegiopoulos
      Pages 37-48
    6. Nicki A. Baker, Lindsey A. Muir, Carey N. Lumeng, Robert W. O’Rourke
      Pages 61-76
  3. Functional Analysis, Bioengineering, and Other Applications

    1. Front Matter
      Pages 87-87
    2. Xingxing Kong, Kevin W. Williams, Tiemin Liu
      Pages 99-107
    3. Lawrence Kazak
      Pages 125-134
    4. Yanhui Zhang, Dan Tong, Anil Mishra, Litao Xie, Isaac Samuel, Jessica K. Smith et al.
      Pages 145-150
    5. Maria Chondronikola, Craig Porter, John O. Ogunbileje, Labros S. Sidossis
      Pages 159-176
    6. Takeshi Akama, Brendan M. Leung, Joe Labuz, Shuichi Takayama, Tae-Hwa Chun
      Pages 177-183
    7. Jessica C. Brooks, Robert L. Judd, Christopher J. Easley
      Pages 185-201
  4. Back Matter
    Pages 213-214

About this book


This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, and differentiate primary fat cells from both laboratory mice and humans. This part also presents flow cytometry methods to isolate various subpopulations of precursors within the stromal vascular fraction of the adipose tissue, which contains both preadipocytes and immune cells. Part 2 introduces multiple means to genetically manipulate and evaluate brown and beige fat in vivo. The chapters in this section explore methods on bioenergetics analyses both in vitro and in vivo. They also cover how to evaluate thermogenic fat contents and activity in humans, how to culture these cells though interdisciplinary approaches, and how to use thermogenic fat cell lines to carry out drug screens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Thermogenic Fat: Methods and Protocols is a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions.


Systematic metabolism Brown fat cells Beige fat cells Energy metabolism Adipocytes

Editors and affiliations

  • Jun Wu
    • 1
  1. 1.Department of Molecular and Integrative Physiology, Life Sciences InstituteUniversity of MichiganAnn ArborUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6819-0
  • Online ISBN 978-1-4939-6820-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site