Light Microscopy

Methods and Protocols

  • Yolanda Markaki
  • Hartmann Harz

Part of the Methods in Molecular Biology book series (MIMB, volume 1563)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Joel Ryan, Abby R. Gerhold, Vincent Boudreau, Lydia Smith, Paul S. Maddox
    Pages 1-15
  3. Advanced Fluorescence Microscopy: Sample Preparation, Fluorophores and Model Systems

    1. Front Matter
      Pages 17-17
    2. Francesco Pampaloni, Laura Knuppertz, Andrea Hamann, Heinz D. Osiewacz, Ernst H. K. Stelzer
      Pages 19-31
    3. Nina Jährling, Klaus Becker, Saiedeh Saghafi, Hans-Ulrich Dodt
      Pages 33-49
    4. Erinke van Grinsven, Chloé Prunier, Nienke Vrisekoop, Laila Ritsma
      Pages 51-71
    5. Peter Quicke, Samuel J. Barnes, Thomas Knöpfel
      Pages 73-84
    6. David Domozych, Anna Lietz, Molly Patten, Emily Singer, Berke Tinaz, Sandra C. Raimundo
      Pages 91-105
    7. Naga Venkata Gayathri Vegesna, Paolo Ronchi, Sevi Durdu, Stefan Terjung, Rainer Pepperkok
      Pages 107-125
  4. Super and High-Resolution Optical Imaging

    1. Front Matter
      Pages 127-127
    2. Sandra Fendl, Jesús Pujol-Martí, Joel Ryan, Alexander Borst, Robert Kasper
      Pages 143-150
    3. Rainer A. Leitgeb
      Pages 167-182
  5. Quantitative and Computational Image Analysis

    1. Front Matter
      Pages 183-183
    2. Ignacio Arganda-Carreras, Philippe Andrey
      Pages 185-207
    3. Yao Yao, Ihor Smal, Ilya Grigoriev, Maud Martin, Anna Akhmanova, Erik Meijering
      Pages 209-228
    4. José Rino, Ana C. de Jesus, Maria Carmo-Fonseca
      Pages 229-242
    5. Nickolaos Nikiforos Giakoumakis, Maria Anna Rapsomaniki, Zoi Lygerou
      Pages 243-267
  6. Back Matter
    Pages 281-285

About this book


This volume addresses up-to-date light microscopy approaches and toolsets offered for live- or fixed-cell observations. The imaging strategies discussed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and laser microsurgery experiments. Chapters also describe the use of these imaging methodologies to study properties of a multitude of biomolecular targets in a broad range of model systems ranging from bacteria over tissue to whole animal imaging. Light Microscopy: Methods and Protocols puts special focus on system instrumentation parameters and sophisticated labeling and detection methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Light Microscopy: Methods and Protocols offers the novice user with straightforward strategies to address biological questions, while providing the experienced researcher with the latest applications that can be useful in routine practices. This book also serves as a useful teaching manual in practical courses on light microscopy.


confocal laser scanning and spinning disk confocal microscopy Fluorescence Resonance Energy Transfer (FRET) Fluorescence Recovery After Photobleaching (FRAP) light-sheet and two-photon excitation microscopy Photoactivated Localization Microscopy (PALM) Stimulated Emission Depletion (STED) Total Internal Reflection Fluorescence (TIRF) biomolecular spatiotemporal dynamics

Editors and affiliations

  • Yolanda Markaki
    • 1
  • Hartmann Harz
    • 2
  1. 1.Department of Biological ChemistryUCLA School of MedicineLos AngelesUSA
  2. 2.Biozentrum der LMU MunchenPlanegg-MartinsriedGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6808-4
  • Online ISBN 978-1-4939-6810-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site