RNA Methylation

Methods and Protocols

  • Alexandra Lusser

Part of the Methods in Molecular Biology book series (MIMB, volume 1562)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Modifications in Abundant RNA Species

    1. Front Matter
      Pages 1-1
    2. Kathrin Thüring, Katharina Schmid, Patrick Keller, Mark Helm
      Pages 3-18
    3. Mellie June Paulines, Patrick A. Limbach
      Pages 19-32
  3. Analysis of N6-Methyladenosine

    1. Front Matter
      Pages 43-43
    2. Benoit Molinie, Cosmas C. Giallourakis
      Pages 45-53
    3. Anya V. Grozhik, Bastian Linder, Anthony O. Olarerin-George, Samie R. Jaffrey
      Pages 55-78
  4. Analysis of 5-Methylcytosine

    1. Front Matter
      Pages 89-89
    2. Thomas Amort, Xueguang Sun, Daria Khokhlova-Cubberley, Alexandra Lusser
      Pages 123-142
    3. Dietmar Rieder, Francesca Finotello
      Pages 143-154
  5. Further RNA Modifications

    1. Front Matter
      Pages 169-169
    2. Virginie Marchand, Lilia Ayadi, Aseel El Hajj, Florence Blanloeil-Oillo, Mark Helm, Yuri Motorin
      Pages 171-187
    3. Nicolai Krogh, Ulf Birkedal, Henrik Nielsen
      Pages 189-209
    4. Pavel P. Kuksa, Yuk Yee Leung, Lee E. Vandivier, Zachary Anderson, Brian D. Gregory, Li-San Wang
      Pages 211-229
    5. Eva Hrabeta-Robinson, Erin Marcus, Aaron E. Cozen, Eric M. Phizicky, Todd M. Lowe
      Pages 231-243
    6. Xiaoyu Li, Jinying Peng, Chengqi Yi
      Pages 245-255
  6. Functional Assays for Modified and Unmodified RNA

    1. Front Matter
      Pages 257-257
    2. Sara Haag, Katherine E. Sloan, Claudia Höbartner, Markus T. Bohnsack
      Pages 259-268
    3. Sara Haag, Jens Kretschmer, Katherine E. Sloan, Markus T. Bohnsack
      Pages 269-281
    4. Thomas P. Hoernes, Matthias D. Erlacher
      Pages 283-294
  7. Back Matter
    Pages 303-304

About this book


This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation. The protocols in this book discuss state-of-the-art methods for investigating aspects of RNA methylation on different types of RNA. The protocols cover topics such as wet-lab techniques for the detection of methylation, instructions for bioinformatics analyses of transcriptome-scale data, and protocols for the functional examination of RNA modifications and enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, RNA Methylation: Methods and Protocols is a valuable resource for biochemists and molecular biologists, from various fields, who wish to investigate different types of RNA methylations.



posttranscriptional base modifications N6-methyladenosine (m6A) 5-methyl cytosine (m5C) mRNAs long noncoding RNA RNA epigenetics analyses of transcriptome-scale data sets enzymes

Editors and affiliations

  • Alexandra Lusser
    • 1
  1. 1.Division of Molecular Biology, BiocenterMedical University of InnsbruckInnsbruckAustria

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6805-3
  • Online ISBN 978-1-4939-6807-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site