SH2 Domains

Methods and Protocols

  • Kazuya Machida
  • Bernard A. Liu

Part of the Methods in Molecular Biology book series (MIMB, volume 1555)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Overview

    1. Front Matter
      Pages 1-1
    2. Bernard A. Liu, Kazuya Machida
      Pages 3-35
    3. Bruce J. Mayer
      Pages 37-43
  3. Computational Approaches

  4. Experimental Tools

    1. Front Matter
      Pages 115-115
    2. Bernard A. Liu, Mari Ogiue-Ikeda, Kazuya Machida
      Pages 117-162
    3. Nicholas P. D. Liau, Artem Laktyushin, Jeffrey J. Babon
      Pages 173-182
    4. Haiming Huang, Tomonori Kaneko, Sachdev S. Sidhu, Shawn S. C. Li
      Pages 225-254
  5. Structural Analysis and Binding Assays

    1. Front Matter
      Pages 255-255
    2. Radu Huculeci, Fabien Kieken, Abel Garcia-Pino, Lieven Buts, Nico van Nuland, Tom Lenaerts
      Pages 257-267
    3. Marissa A. McKercher, Deborah S. Wuttke
      Pages 269-290
    4. Marissa A. McKercher, Deborah S. Wuttke
      Pages 291-305
  6. High-Throughput Screening and Proteomics

  7. Functional Analysis

  8. Back Matter
    Pages 547-555

About this book


This volume covers an array of techniques available for studying SH2 domains and phosphotyrosine signaling. The book is divided into six parts: Part I outlines the history of SH2, technology development, and cell signaling; Part II focuses on computational approaches and tools used for identification, classification, and predictions of SH2 domain binding partners; Part III details various ways to prepare the SH2 domains as experimental reagents; Part IV presents methods for structural analysis and conventional binding assays using SH2 domains; Part V describes high-throughput and proteomics approaches to aid in analyzing SH2-mediated interactions; and Part VI covers applications for SH2 domain to functional and imaging analyzes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, SH2 Domains: Methods and Protocols is a valuable resource for computational biologists, biochemists, structural biologists, cell biologists, pathologists, and people interested in SH2 domains and phosphotyrosine signaling. Researchers who are investigating how protein interaction domain mediate specificity in signaling systems may also find this book informative.


receptor tyrosine kinases (RTKs) phosphotyrosine signaling cell signaling structural analysis conventional binding assays high-throughput and proteomics approaches SH2-mediated interactions

Editors and affiliations

  • Kazuya Machida
    • 1
  • Bernard A. Liu
    • 2
  1. 1.Department of Genetics and Genome SciencesUniversity of Connecticut School of MedicineFarmingtonUSA
  2. 2.Broad Institute of MIT and HarvardCambridgeUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6760-5
  • Online ISBN 978-1-4939-6762-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site