Methods and Protocols

  • Irene Tiemann-Boege
  • Andrea Betancourt

Part of the Methods in Molecular Biology book series (MIMB, volume 1551)

Table of contents

  1. Front Matter
    Pages i-x
  2. Haplotyping with Long Range PCR

    1. Front Matter
      Pages 1-1
    2. Barbara Arbeithuber, Angelika Heissl, Irene Tiemann-Boege
      Pages 3-22
    3. Kyuha Choi, Nataliya E. Yelina, Heïdi Serra, Ian R. Henderson
      Pages 23-57
  3. Selective Sequencing of Gene Families (including the MHC)

    1. Front Matter
      Pages 59-59
    2. Wei Zhang, Joachim Messing
      Pages 61-71
    3. Nicholas M. Murphy, Colin W. Pouton, Helen R. Irving
      Pages 73-85
    4. Johannes Pröll, Carina Fischer, Gabriele Michelitsch, Martin Danzer, Norbert Niklas
      Pages 87-112
    5. Chester A. Alper, Charles E. Larsen
      Pages 113-127
  4. Haploid-cell Typing

  5. Haplotyping Single Chromosomes

    1. Front Matter
      Pages 159-159
    2. Xi Chen, Hong Yang, Wing Hung Wong
      Pages 171-188
  6. Genome Wide Haplotyping

    1. Front Matter
      Pages 189-189
    2. Mark A. McElwain, Rebecca Yu Zhang, Radoje Drmanac, Brock A. Peters
      Pages 191-205
    3. Lena Christiansen, Sasan Amini, Fan Zhang, Mostafa Ronaghi, Kevin L. Gunderson, Frank J. Steemers
      Pages 207-221
    4. Eun-Kyung Suk, Sabrina Schulz, Birgit Mentrup, Thomas Huebsch, Jorge Duitama, Margret R. Hoehe
      Pages 223-269
  7. High throughput Haplotyping of Rare Variants

    1. Front Matter
      Pages 271-271
    2. Elisabeth Palzenberger, Ronja Reinhardt, Leila Muresan, Barbara Palaoro, Irene Tiemann-Boege
      Pages 273-305
  8. Computational Algorithms for Haplotyping

    1. Front Matter
      Pages 307-307
  9. Back Matter
    Pages 321-325

About this book


This volume details protocols for genetic, molecular, cytological, and bioinformatic methods for determining haplotypes.  Haplotyping: Methods and Protocols guides readers through methods that directly type haploid cells, difficult-to-resolve gene families, high-resolution, short range haplotyping for targeted loci, and long-range haplotyping for whole chromosomes or genomes.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical Haplotyping: Methods and Protocols, aims to provide researchers with an overview of experimental methods for haplotyping.


chromosome allelic phasing haplotype phasing cytological bioinformatic methods short range haplotyping long-range haplotyping genomes

Editors and affiliations

  • Irene Tiemann-Boege
    • 1
  • Andrea Betancourt
    • 2
  1. 1.Johannes Kepler University Institute of BiophysicsLinzAustria
  2. 2.Vetmeduni Vienna InstitutPopulationsgenetikWienAustria

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6748-3
  • Online ISBN 978-1-4939-6750-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site