Book Volume 1551 2017

Haplotyping

Methods and Protocols

Editors:

ISBN: 978-1-4939-6748-3 (Print) 978-1-4939-6750-6 (Online)

Table of contents (15 protocols)

  1. Front Matter

    Pages i-x

  2. Haplotyping with Long Range PCR

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-22

      Haplotyping of Heterozygous SNPs in Genomic DNA Using Long-Range PCR

    3. No Access

      Protocol

      Pages 23-57

      Quantification and Sequencing of Crossover Recombinant Molecules from Arabidopsis Pollen DNA

  3. Selective Sequencing of Gene Families (including the MHC)

    1. Front Matter

      Pages 59-59

    2. No Access

      Protocol

      Pages 61-71

      PacBio for Haplotyping in Gene Families

    3. No Access

      Protocol

      Pages 73-85

      High Molecular Weight DNA Enrichment with Peptide Nucleic Acid Probes

    4. No Access

      Protocol

      Pages 87-112

      High-Throughput Sequencing of the Major Histocompatibility Complex following Targeted Sequence Capture

    5. No Access

      Protocol

      Pages 113-127

      Pedigree-Defined Haplotypes and Their Applications to Genetic Studies

  4. Haploid-cell Typing

    1. Front Matter

      Pages 129-129

    2. No Access

      Protocol

      Pages 131-146

      Haplotyping a Non-meiotic Diploid Fungal Pathogen Using Induced Aneuploidies and SNP/CGH Microarray Analysis

    3. No Access

      Protocol

      Pages 147-157

      Whole-Genome Haplotyping of Single Sperm of Daphnia pulex (Crustacea, Anomopoda)

  5. Haplotyping Single Chromosomes

    1. Front Matter

      Pages 159-159

    2. No Access

      Protocol

      Pages 161-169

      Chromosome-Range Whole-Genome High-Throughput Experimental Haplotyping by Single-Chromosome Microdissection

    3. No Access

      Protocol

      Pages 171-188

      Phased Genome Sequencing Through Chromosome Sorting

  6. Genome Wide Haplotyping

    1. Front Matter

      Pages 189-189

    2. No Access

      Protocol

      Pages 191-205

      Long Fragment Read (LFR) Technology: Cost-Effective, High-Quality Genome-Wide Molecular Haplotyping

    3. No Access

      Protocol

      Pages 207-221

      Contiguity-Preserving Transposition Sequencing (CPT-Seq) for Genome-Wide Haplotyping, Assembly, and Single-Cell ATAC-Seq

    4. No Access

      Protocol

      Pages 223-269

      A Fosmid Pool-Based Next Generation Sequencing Approach to Haplotype-Resolve Whole Genomes

  7. High throughput Haplotyping of Rare Variants

    1. Front Matter

      Pages 271-271

    2. No Access

      Protocol

      Pages 273-305

      Discovery of Rare Haplotypes by Typing Millions of Single-Molecules with Bead Emulsion Haplotyping (BEH)

  8. Computational Algorithms for Haplotyping

    1. Front Matter

      Pages 307-307

    2. No Access

      Protocol

      Pages 309-319

      Computational Haplotype Inference from Pooled Samples

  9. Back Matter

    Pages 321-325