Book Volume 1547 2017

Microchip Diagnostics

Methods and Protocols

Editors:

ISBN: 978-1-4939-6732-2 (Print) 978-1-4939-6734-6 (Online)

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi

  2. Part I

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-21

      Microfluidics-Enabled Diagnostic Systems: Markets, Challenges, and Examples

  3. Microchips for Protein Bioassays

    1. Front Matter

      Pages 23-23

    2. No Access

      Protocol

      Pages 25-36

      Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Efficient Fabrication and Sealing of Chips Using a “Chip-Olate” Process

    3. No Access

      Protocol

      Pages 37-47

      Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Patterning Capture Antibodies Using Microcontact Printing and Dry-Film Resists

    4. No Access

      Protocol

      Pages 49-56

      Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars

    5. No Access

      Protocol

      Pages 57-67

      Using Electrophoretic Immunoassay to Monitor Hormone Secretion

    6. No Access

      Protocol

      Pages 69-83

      On-Chip Magnetic Particle-Based Immunoassays Using Multilaminar Flow for Clinical Diagnostics

    7. No Access

      Protocol

      Pages 85-101

      Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in Femtoliter-Sized Reaction Wells for Single-Molecule Detection

  4. Microchips for Nucleic Acids Analysis

    1. Front Matter

      Pages 103-103

    2. No Access

      Protocol

      Pages 105-111

      Microfluidic DNA Stretching Device for Single-Molecule Diagnostics

    3. No Access

      Protocol

      Pages 113-121

      Droplet Microfluidic and Magnetic Particles Platform for Cancer Typing

    4. No Access

      Protocol

      Pages 123-132

      SlipChip Device for Digital Nucleic Acid Amplification

    5. No Access

      Protocol

      Pages 133-142

      Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion

    6. No Access

      Protocol

      Pages 143-164

      Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations

  5. Microfluidic Chips for Cell-Based Enrichment and Analysis

    1. Front Matter

      Pages 165-165

    2. No Access

      Protocol

      Pages 167-174

      Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic Chip

    3. No Access

      Protocol

      Pages 175-186

      Microfluidic-Based Bacteria Isolation from Whole Blood for Diagnostics of Blood Stream Infection

    4. No Access

      Protocol

      Pages 187-209

      Microfluidic Platform for Parallel Single Cell Analysis for Diagnostic Applications

    5. No Access

      Protocol

      Pages 211-220

      FISH-in-CHIPS: A Microfluidic Platform for Molecular Typing of Cancer Cells

  6. Back Matter

    Pages 221-223