Book Volume 1544 2017

Xylem

Methods and Protocols

Editors:

ISBN: 978-1-4939-6720-9 (Print) 978-1-4939-6722-3 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-x

  2. Development

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-19

      Experimental and Theoretical Methods to Approach the Study of Vascular Patterning in the Plant Shoot

    3. No Access

      Protocol

      Pages 21-26

      Strigolactone-mediated Stimulation of Secondary Xylem Proliferation in Stems

    4. No Access

      Protocol

      Pages 27-36

      Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues

    5. No Access

      Protocol

      Pages 37-57

      Establishment and Utilization of Habituated Cell Suspension Cultures for Hormone-Inducible Xylogenesis

    6. No Access

      Protocol

      Pages 59-65

      Tissue Culture for Xylem Differentiation with Arabidopsis Leaves

    7. No Access

      Protocol

      Pages 67-73

      VND6-induced Xylem Cell Differentiation in Arabidopsis Cell Cultures

  3. Imaging

    1. Front Matter

      Pages 75-75

    2. No Access

      Protocol

      Pages 77-82

      Live Imaging of Developing Xylem In Planta

    3. No Access

      Protocol

      Pages 83-90

      Immunolocalization in Secondary Xylem of Populus

    4. No Access

      Protocol

      Pages 91-102

      Monitoring Vascular Regeneration and Xylem Connectivity in Arabidopsis thaliana

    5. No Access

      Protocol

      Pages 103-125

      Vascular Morphodynamics During Secondary Growth

    6. No Access

      Protocol

      Pages 127-132

      Xylem Characterization Using Improved Pseudo-Schiff Propidium Iodide Staining of Whole Mount Samples and Confocal Laser-Scanning Microscopy

    7. No Access

      Protocol

      Pages 133-178

      Chemical Imaging of Xylem by Raman Microspectroscopy

    8. No Access

      Protocol

      Pages 179-189

      Using CellProfiler to Analyze and Quantify Vascular Morphology

  4. Composition

    1. Front Matter

      Pages 191-191

    2. No Access

      Protocol

      Pages 193-211

      Lignin Analysis by HPLC and FTIR

    3. No Access

      Protocol

      Pages 213-222

      Carbohydrate Composition Analysis in Xylem

    4. No Access

      Protocol

      Pages 223-231

      Structural Analysis of Cell Wall Polysaccharides Using PACE

    5. No Access

      Protocol

      Pages 233-247

      Analysis of Lignin Composition and Distribution Using Fluorescence Laser Confocal Microspectroscopy

    6. No Access

      Protocol

      Pages 249-256

      Topochemical Analysis of Cell Wall Components by TOF-SIMS

  5. Back Matter

    Pages 257-259