Book Volume 1539 2017

Metagenomics

Methods and Protocols

Editors:

ISBN: 978-1-4939-6689-9 (Print) 978-1-4939-6691-2 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi

  2. No Access

    Protocol

    Pages 1-12

    Construction of Small-Insert and Large-Insert Metagenomic Libraries

  3. No Access

    Protocol

    Pages 13-22

    Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a cDNA as Universal Template for Marker Gene Studies

  4. No Access

    Protocol

    Pages 23-42

    Construction and Screening of Marine Metagenomic Large Insert Libraries

  5. No Access

    Protocol

    Pages 43-55

    Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment

  6. No Access

    Protocol

    Pages 57-74

    DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms

  7. No Access

    Protocol

    Pages 75-84

    Assessing Bacterial and Fungal Diversity in the Plant Endosphere

  8. No Access

    Protocol

    Pages 85-97

    Shotgun Metagenomic Sequencing Analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities

  9. No Access

    Protocol

    Pages 99-144

    Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production

  10. No Access

    Protocol

    Pages 145-157

    Degradation Network Reconstruction Guided by Metagenomic Data

  11. No Access

    Protocol

    Pages 159-196

    Novel Tools for the Functional Expression of Metagenomic DNA

  12. No Access

    Protocol

    Pages 197-204

    A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries

  13. No Access

    Protocol

    Pages 205-217

    Screening for Cellulase Encoding Clones in Metagenomic Libraries

  14. No Access

    Protocol

    Pages 219-228

    Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries Using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes

  15. No Access

    Protocol

    Pages 229-236

    Screening Glycosyltransferases for Polyphenol Modifications

  16. No Access

    Protocol

    Pages 237-248

    Methods for the Isolation of Genes Encoding Novel PHA Metabolism Enzymes from Complex Microbial Communities

  17. No Access

    Protocol

    Pages 249-260

    Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity

  18. No Access

    Protocol

    Pages 261-270

    Activity-Based Screening of Metagenomic Libraries for Hydrogenase Enzymes

  19. No Access

    Protocol

    Pages 271-286

    Screening for N-AHSL-Based-Signaling Interfering Enzymes

  20. No Access

    Protocol

    Pages 287-300

    Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites

  21. Protocol

    Pages E1-E1

    Erratum to: Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production

  22. Back Matter

    Pages 301-303