Methods and Protocols

  • Sebastian Gasparis

Part of the Methods in Molecular Biology book series (MIMB, volume 1536)

Table of contents

  1. Front Matter
    Pages i-x
  2. Cytological Methods

    1. Front Matter
      Pages 1-1
    2. Philip A. Davies, Parminder K. Sidhu
      Pages 23-30
    3. Toshinobu Morikawa
      Pages 43-52
  3. In Vitro Cultures and Genetic Transformation

    1. Front Matter
      Pages 53-53
    2. Robyn Roberts, Jincan Zhang, Nicole Mihelich, Danielle Savino, Aurélie M. Rakotondrafara
      Pages 55-70
    3. Elina Kiviharju, Sirpa Moisander, Pirjo Tanhuanpää
      Pages 71-93
  4. Quality Assessment

    1. Front Matter
      Pages 113-113
    2. Gracia Montilla-Bascón, Corey D. Broeckling, Owen A. Hoekenga, Elena Prats, Mark Sorrells, Julio Isidro-Sánchez
      Pages 115-125
    3. Susanna Oswald, Richard Dietrich, Erwin Märtlbauer, Reinhard Niessner, Dietmar Knopp
      Pages 143-156
  5. Genomic Studies

    1. Front Matter
      Pages 157-157
    2. Julio Isidro-Sánchez, Deniz Akdemir, Gracia Montilla-Bascón
      Pages 189-207
    3. Juan J. Gutierrez-Gonzalez, David F. Garvin
      Pages 209-221
  6. Proteomics Methods

    1. Front Matter
      Pages 223-223
    2. Dorota Nałęcz, Iwona Szerszunowicz, Marta Dziuba, Piotr Minkiewicz
      Pages 235-251
  7. Back Matter
    Pages 271-272

About this book


The volume provides detailed protocols that have been developed or modified exclusively for the study of oat. The topics discussed in this book are a selection of various molecular biology and biotechnology methods, such as the application of molecular markers for polymorphism analyses and cytological manipulations, the production of synthetic polyploids, and in vitro cultures and genetic modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Oat: Methods and Protocols is a useful resource in the development of new research approaches toward organizing the oat genome and the identification of new and useful traits for further improvements of this exceptional crop.


Avenae Hexaploid oat amino acids genetic engineering techniques polymorphism analyses cytological manipulations production of synthetic polyploids in vitro cultures genetic modification genome

Editors and affiliations

  • Sebastian Gasparis
    • 1
  1. 1.Department of Functional GenomicsPlant Breeding & Acclimatization Inst. National Research InstituteBloniePoland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6680-6
  • Online ISBN 978-1-4939-6682-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site