Book Volume 1536 2017

Oat

Methods and Protocols

Editors:

ISBN: 978-1-4939-6680-6 (Print) 978-1-4939-6682-0 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-x

  2. Cytological Methods

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-21

      Fluorescence In Situ Hybridization in Oat

    3. No Access

      Protocol

      Pages 23-30

      Oat Doubled Haploids Following Maize Pollination

    4. No Access

      Protocol

      Pages 31-42

      Wide Hybridization Between Oat and Pearl Millet

    5. No Access

      Protocol

      Pages 43-52

      Protocol for Producing Synthetic Polyploid Oats

  3. In Vitro Cultures and Genetic Transformation

    1. Front Matter

      Pages 53-53

    2. No Access

      Protocol

      Pages 55-70

      Manipulation of Oat Protoplasts for Transient Expression Assays

    3. No Access

      Protocol

      Pages 71-93

      Oat Anther Culture and Use of DH-Lines for Genetic Mapping

    4. No Access

      Protocol

      Pages 95-111

      Agrobacterium-Mediated Transformation of Leaf Base Segments

  4. Quality Assessment

    1. Front Matter

      Pages 113-113

    2. No Access

      Protocol

      Pages 115-125

      Chromatographic Methods to Evaluate Nutritional Quality in Oat

    3. No Access

      Protocol

      Pages 127-136

      Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry

    4. No Access

      Protocol

      Pages 137-142

      Multiplex Dipstick Immunoassay for Semiquantitative Determination of Fusarium Mycotoxins in Oat

    5. No Access

      Protocol

      Pages 143-156

      Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat

  5. Genomic Studies

    1. Front Matter

      Pages 157-157

    2. No Access

      Protocol

      Pages 159-168

      M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat Germplasm

    3. No Access

      Protocol

      Pages 169-187

      Genotyping-by-Sequencing and Its Application to Oat Genomic Research

    4. No Access

      Protocol

      Pages 189-207

      Genome-Wide Association Analysis Using R

    5. No Access

      Protocol

      Pages 209-221

      De Novo Transcriptome Assembly in Polyploid Species

  6. Proteomics Methods

    1. Front Matter

      Pages 223-223

    2. No Access

      Protocol

      Pages 225-234

      Isolation of Oat (Avena sativa L.) Total Proteins and Their Prolamin Fractions for 2D Electrophoresis

    3. No Access

      Protocol

      Pages 235-251

      2-DE Separation and Identification of Oat (Avena sativa L.) Proteins and Their Prolamin Fractions

    4. No Access

      Protocol

      Pages 253-270

      Selected Bioinformatic Tools and MS (MALDI-TOF, PMF) Techniques Used in the Strategy for the Identification of Oat Proteins After 2-DE

  7. Back Matter

    Pages 271-272