Bacterial Pathogenesis

Methods and Protocols

  • Pontus Nordenfelt
  • Mattias Collin

Part of the Methods in Molecular Biology book series (MIMB, volume 1535)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Identification and Characterization of Bacterial Effector Molecules

  3. Structural Biology of Bacterial–Host Interactions

  4. Genetics and Phylogenetics of Bacterial Pathogens

  5. Bacterial Imaging Approaches and Related Techniques

    1. Front Matter
      Pages 139-139
    2. Sarah Shabayek, Barbara Spellerberg
      Pages 141-159
    3. Jonas Danielsen, Pontus Nordenfelt
      Pages 161-172
    4. Juan J. Quereda, Martin Sachse, Damien Balestrino, Théodore Grenier, Jennifer Fredlund, Anne Danckaert et al.
      Pages 173-195
    5. Amy L. Bottomley, Lynne Turnbull, Cynthia B. Whitchurch, Elizabeth J. Harry
      Pages 197-209
    6. Kenneth L. Pinkston, Peng Gao, Kavindra V. Singh, Ali Azhdarinia, Barbara E. Murray, Eva M. Sevick-Muraca et al.
      Pages 229-241
  6. Models for Studying Bacterial Pathogenesis

    1. Front Matter
      Pages 243-243
    2. Diego López León, Isabelle Fellay, Pierre-Yves Mantel, Michael Walch
      Pages 275-284
    3. Yashuan Chao, Caroline Bergenfelz, Anders P. Håkansson
      Pages 285-299
    4. Julia Lienard, Fredric Carlsson
      Pages 301-315
  7. Methods Exploiting Bacterial Immune Evasion

    1. Front Matter
      Pages 317-317
    2. Jonathan Sjögren, Linda Andersson, Malin Mejàre, Fredrik Olsson
      Pages 319-329
    3. Oonagh Shannon, Pontus Nordenfelt
      Pages 331-337
  8. Back Matter
    Pages 353-357

About this book


This volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such as Streptococcus pyogenes, Staphylococcus aureus, Streptococcus pneumonia, Listeria monocytogenes, and Mycobacterium marinum. Bacterial Pathogens: Methods and Protocols is divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals with structural biology of bacterial pathogenesis and how to overcome folding and stability problems with recombinantly expressed proteins; Part 3 details methodology that identifies bacteria in complex communities and how genomes of bacterial pathogens have evolved; Part 4 reflects on the rapid development of advanced imaging techniques that address questions about molecular properties of individual live bacteria, ultrastructure of surfaces, and subcellular localization of bacterial proteins; Part 5 describes methods from in vitro and in vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Bacterial Pathogens: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.


streptococcus pyogenes staphylococcus aureus streptococcus pneumoniae listeria monocytogenes escherichia coli salmonella typhimurium subcellular localization of bacterial proteins zebrafish immunoglobulin active enzymes antibody-mediated diseases

Editors and affiliations

  • Pontus Nordenfelt
    • 1
  • Mattias Collin
    • 2
  1. 1.Department of Clinical SciencesLund University, Division of Infection MedicineLundSweden
  2. 2.Department of Clinical SciencesLund University, Division of Infection MedicineLundSweden

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6671-4
  • Online ISBN 978-1-4939-6673-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site