Table of contents

  1. Front Matter
    Pages i-xii
  2. Matthew Gray, Roberto J. Botelho
    Pages 1-16
  3. Noah Fine, Oriyah Barzilay, Michael Glogauer
    Pages 17-24
  4. Amanda L. Evans, Jack W. D. Blackburn, Charles Yin, Bryan Heit
    Pages 25-41
  5. Christopher H. Choy, Roberto J. Botelho
    Pages 43-53
  6. Stella M. Lu, Sergio Grinstein, Gregory D. Fairn
    Pages 79-91
  7. Shashank Shekhar, Vinod Subramaniam, Johannes S. Kanger
    Pages 93-112
  8. Roya M. Dayam, Roberto J. Botelho
    Pages 113-123
  9. Laure-Anne Ligeon, Susana Romao, Christian Münz
    Pages 145-168
  10. Laura Schnettger, Maximiliano G. Gutierrez
    Pages 169-184
  11. Johnathan Canton, Sergio Grinstein
    Pages 185-199
  12. Nicholas D. Condon, Adam A. Wall, Jeremy C. Yeo, Nicholas A. Hamilton, Jennifer L. Stow
    Pages 201-214
  13. Anetta Härtlova, Julien Peltier, Orsolya Bilkei-Gorzo, Matthias Trost
    Pages 241-248
  14. Julien Peltier, Anetta Härtlova, Matthias Trost
    Pages 249-263
  15. Shiya Cheng, Kai Liu, Chonglin Yang, Xiaochen Wang
    Pages 265-284
  16. Jessica A. Klein, TuShun R. Powers, Leigh A. Knodler
    Pages 285-296
  17. Kyle E. Novakowski, Dessi Loukov, Vikash Chawla, Dawn M. E. Bowdish
    Pages 297-309
  18. Akriti Prashar, Sana I. S. Khan, Mauricio R. Terebiznik
    Pages 311-323
  19. Evgeniya V. Nazarova, David G. Russell
    Pages 325-331
  20. Robert J. Evans, Kerstin Voelz, Simon A. Johnston, Robin C. May
    Pages 349-357
  21. Back Matter
    Pages 359-360

About this book


This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and protocols on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Phagocytosis and Phagosomes: Methods and Protocols aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time.


phagocytes pathogens phagosome maturation cell fractionation Western blotting proteomics

Editors and affiliations

  • Roberto Botelho
    • 1
  1. 1.Department of Chemistry and BiologyRyerson UniversityTorontoCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6579-3
  • Online ISBN 978-1-4939-6581-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site