Cohesin and Condensin

Methods and Protocols

  • Kyoko Yokomori
  • Katsuhiko Shirahige

Part of the Methods in Molecular Biology book series (MIMB, volume 1515)

Table of contents

  1. Front Matter
    Pages i-xii
  2. In Vitro and In Vivo Systems to Dissect Molecular Mechanisms of Cohesin Regulation in Mitosis and Meiosis

    1. Front Matter
      Pages 1-1
    2. Keishi Shintomi, Tatsuya Hirano
      Pages 3-21
    3. Ge Zheng, Zhuqing Ouyang, Hongtao Yu
      Pages 37-53
    4. Marcelino Bermúdez-López, Luis Aragón
      Pages 55-64
    5. Michelle Stevense, Rolf Jessberger, Attila Tóth
      Pages 97-111
  3. The Roles of Cohesin in Gene Regulation, Chromatin Interactions, Ribosome Biogenesis, and DNA Repair

    1. Front Matter
      Pages 113-113
    2. Misty R. Peterson, Omar Hamdani, Rohinton T. Kamakaka
      Pages 151-176
    3. Akihiko Muto, Thomas F. Schilling
      Pages 177-196
    4. Preksha Gupta, Thais Lavagnolli, Hegias Mira-Bontenbal, Matthias Merkenschlager
      Pages 197-216
    5. Xiangduo Kong, Alexander R. Ball Jr., Kyoko Yokomori
      Pages 227-242
  4. Dissection of Condensin Function in Mitotic Chromosome Condensation and Gene Regulation

    1. Front Matter
      Pages 243-243
    2. Christoph Schiklenk, Boryana Petrova, Christian H. Haering
      Pages 245-255
    3. Toyonori Sakata, Katsuhiko Shirahige, Takashi Sutani
      Pages 257-271
  5. Back Matter
    Pages 273-276

About this book


The volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. Cohesin and Condensin: Methods and Protocols is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fundamental mechanism of cohesin regulation in mitosis and meiosis; Part II summarizes experimental systems in a variety of organisms that are used to address interphase functions of cohesin and Nipbl in gene regulation and chromatin interaction, ribosome biogenesis and DNA repair, which contribute significantly to cohesion-associated disorders; Part III covers related condensin complex and describes techniques to study its role in mitosis and interphase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cohesin and Condensin: Methods and Protocols is a valuable resource for diverse audiences with interests in the relationship between chromatin organization and genomic functions.


cohesin regulation cell-free system in Xenopus cohesin-dependent sister chromatid cohesion loading factor Scc2 (Nipbl) destabilizer Wapl SUMO modification DNA mitosis and meiosis

Editors and affiliations

  • Kyoko Yokomori
    • 1
  • Katsuhiko Shirahige
    • 2
  1. 1.Department of Biological ChemistrySchool of Medicine University of California— IrvineIrvineUSA
  2. 2.Laboratory of Genome Structure and Function Research Center for Epigenetic DiseaseInstitute of Molecular and Cellular Biosciences The University of TokyoTokyoJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6543-4
  • Online ISBN 978-1-4939-6545-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site