HDAC/HAT Function Assessment and Inhibitor Development

Methods and Protocols

  • Oliver H. Krämer

Part of the Methods in Molecular Biology book series (MIMB, volume 1510)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Analysis of Cellular Processes That Are Regulated by HDACs

    1. Front Matter
      Pages 1-1
    2. Anja Göder, Nisintha Mahendrarajah, Oliver H. Krämer
      Pages 3-10
    3. Fabian Treude, Tobias Gladbach, Jacqueline Plaster, Jörg Hartkamp
      Pages 11-22
    4. Lisa Marx-Blümel, Christian Marx, Marie Kühne, Jürgen Sonnemann
      Pages 23-45
    5. Britta Unruhe-Knauf, Shirley K. Knauer
      Pages 47-59
    6. Darren M. Hutt, Daniela Martino Roth, Christelle Marchal, Marion Bouchecareilh
      Pages 77-91
    7. René Winkler, Christian Kosan
      Pages 93-101
    8. Teodora Nikolova, Anja Göder, Ann Parplys, Kerstin Borgmann
      Pages 103-113
  3. Primary Cell Systems and In Vivo Models to Assess HDAC Functions

    1. Front Matter
      Pages 125-125
    2. Thierry Buchou, Minjia Tan, Sophie Barral, Anne-Laure Vitte, Sophie Rousseaux, Juan Arechaga et al.
      Pages 159-168
    3. Astrid Hagelkruys, Mirjam A. Moser, Christian Seiser
      Pages 169-192
    4. Christina Susanne Mullins, Stephanie Bock, Mathias Krohn, Michael Linnebacher
      Pages 217-229
  4. Assessment of HDAC-Dependent Effects on Signaling Cascades in Primary and Permanent Cells

  5. PTMs Controlling Acetylation Balances and HDAC Functions

    1. Front Matter
      Pages 295-295
    2. Jan Hagenbucher, Hilda Stekman, Alfonso Rodriguez-Gil, Michael Kracht, M. Lienhard Schmitz
      Pages 297-312
    3. Martin Wieczorek, Karl-Heinz Gührs, Thorsten Heinzel
      Pages 313-327
    4. Simona Citro, Susanna Chiocca
      Pages 329-337
    5. Tobias Wagner, Maren Godmann, Thorsten Heinzel
      Pages 339-351
  6. Improved, Novel, and Specific HDACi

    1. Front Matter
      Pages 353-353
    2. Mandy Beyer, Nicole Kiweler, Siavosh Mahboobi, Oliver H. Krämer
      Pages 355-364
    3. Siavosh Mahboobi, Bernadette Pilsl, Andreas Sellmer
      Pages 405-412
  7. Back Matter
    Pages 413-415

About this book


This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step by step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, HDAC HAT Function Assessment and Inhibitor Development: Methods and Protocols serves as an ideal guide to this vital area of study.


Reversible protein acetylation Deacetylase inhibitors Histone deacetylases Histone acetyltransferases Protein function

Editors and affiliations

  • Oliver H. Krämer
    • 1
  1. 1.Institute of ToxicologyUniversity Medical Center MainzMainzGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6525-0
  • Online ISBN 978-1-4939-6527-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site