Enzyme Stabilization and Immobilization

Methods and Protocols

  • Shelley D. Minteer

Part of the Methods in Molecular Biology book series (MIMB, volume 1504)

Table of contents

  1. Front Matter
    Pages i-x
  2. Michael J. Moehlenbrock, Shelley D. Minteer
    Pages 1-7
  3. Keith J. Stine, Kenise Jefferson, Olga V. Shulga
    Pages 37-60
  4. Glenn R. Johnson, Heather R. Luckarift
    Pages 61-73
  5. Zorica D. Knežević-Jugović, Sanja Ž. Grbavčić, Jelena R. Jovanović, Andrea B. Stefanović, Dejan I. Bezbradica, Dušan Ž. Mijin et al.
    Pages 75-91
  6. Sabina Besic, Shelley D. Minteer
    Pages 93-108
  7. Ipsita Roy, Joyeeta Mukherjee, Munishwar N. Gupta
    Pages 109-123
  8. Nenad B. Milosavić, Radivoje M. Prodanović, Dušan Velićković, Aleksandra Dimitrijević
    Pages 139-147
  9. Amanda S. Harper-Leatherman, Jean Marie Wallace, Debra R. Rolison
    Pages 149-163
  10. Gaige R. VandeZande, Jasmine M. Olvany, Julia L. Rutherford, Michelle Rasmussen
    Pages 165-179
  11. Michael J. Cooney
    Pages 215-232
  12. Back Matter
    Pages 233-234

About this book

Introduction

This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).

Keywords

liposome formation micelle introduction crosslinking sol-gel encapsulation polymer encapsulation single enzyme nanoparticle formation spectroscopic protocols

Editors and affiliations

  • Shelley D. Minteer
    • 1
  1. 1.Department of Chemistry, and Department of Materials Science and EngineeringUniversity of UtahSalt Lake CityUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6499-4
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6497-0
  • Online ISBN 978-1-4939-6499-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book