RNA Vaccines

Methods and Protocols

  • Thomas Kramps
  • Knut Elbers

Part of the Methods in Molecular Biology book series (MIMB, volume 1499)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Thomas Kramps, Knut Elbers
    Pages 1-11
  3. Self-Replicating RNA Vectors

    1. Front Matter
      Pages 13-13
    2. Birke Andrea Tews, Gregor Meyers
      Pages 15-35
    3. Thomas Démoulins, Pavlos C. Englezou, Panagiota Milona, Nicolas Ruggli, Nicola Tirelli, Chantal Pichon et al.
      Pages 37-75
    4. Yiyang Zhou, Alison A. McCormick, Christopher M. Kearney
      Pages 77-86
  4. Non-replicating mRNA Vectors

    1. Front Matter
      Pages 87-87
    2. Susanne Rauch, Johannes Lutz, Aleksandra Kowalczyk, Thomas Schlake, Regina Heidenreich
      Pages 89-107
    3. Norbert Pardi, Drew Weissman
      Pages 109-121
    4. Richard Weiss, Sandra Scheiblhofer, Josef Thalhamer
      Pages 123-139
  5. Adjuvantation and Delivery

    1. Front Matter
      Pages 141-141
    2. Norbert Pardi, Drew Weissman
      Pages 143-153
    3. Lukasz Bialkowski, Kevin Van der Jeught, Dries Renmans, Alexia van Weijnen, Carlo Heirman, Marleen Keyaerts et al.
      Pages 179-191
    4. Kate E. Broderick, Laurent M. Humeau
      Pages 193-200
  6. Preclinical and Clinical Development

    1. Front Matter
      Pages 201-201
    2. Thomas Hinz, Kajo Kallen, Cedrik M. Britten, Bruno Flamion, Ulrich Granzer, Axel Hoos et al.
      Pages 203-222
    3. Mustafa Diken, Mathias Vormehr, Christian Grunwitz, Sebastian Kreiter, Özlem Türeci, Ugur Sahin
      Pages 223-236
    4. Gundel Hager
      Pages 253-272
    5. Mirko Kummer, Beatrice Schuler-Thurner
      Pages 273-278
  7. Back Matter
    Pages 279-282

About this book


This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinical development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, RNA Vaccines: Methods and Protocols aims to increased collaboration on RNA vaccines between basic and applied scientists in academia, government, and industry to develop future solutions for today’s challenges.


recombinant viruses self-amplifying mRNA non-replicating mRNA vectors vector platforms viruses

Editors and affiliations

  • Thomas Kramps
    • 1
  • Knut Elbers
    • 2
  1. 1.Boehringer Ingelheim Pharma GmbH & Co. KGIngelheim am RheinGermany
  2. 2.Boehringer Ingelheim GmbHIngelheim am RheinGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6479-6
  • Online ISBN 978-1-4939-6481-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site