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CCN Proteins

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Masaharu Takigawa

    1. Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama, Japan

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1489)

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Table of contents (46 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. The CCN Proteins: An Overview

    • Masaharu Takigawa
    Pages 1-8

  3. Basic Methods and Protocols

    1. Front Matter

      Pages 9-9
      Download chapter PDF

    2. Gene Expression Analysis of CCN Proteins: Whole-Mount In Situ Hybridization of Ccn2 in Developing Calcified Tissues

      • Tomoichiro Yamaai, Masaharu Takigawa
      Pages 11-19

    3. Expression of CCN Genes and Proteins in Human Skin: Methods and Protocols

      • Yilei Cui, Sarah Campbell, Gary J. Fisher
      Pages 21-31

    4. Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cells

      • Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa
      Pages 33-41

    5. Western Blotting Analysis of CCN Proteins in Calcified Tissues

      • Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa
      Pages 43-51

    6. Immunohistochemical Analysis of CCN Proteins in Calcified Tissues

      • Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa
      Pages 53-62

    7. Analysis of CCN Expression by Immunofluorescence on Skin Cells, Skin, and Reconstructed Epidermis

      • Muriel Cario-Andre
      Pages 63-76

    8. Production of Recombinant CCN2 Protein in Escherichia coli

      • Eriko Aoyama, Takako Hattori, Satoshi Kubota, Masaharu Takigawa
      Pages 77-84

    9. Production of Recombinant CCN Proteins by Brevibacillus choshinensis

      • Hiroshi Hanagata, Makoto Mizukami
      Pages 85-93

    10. Production of Recombinant CCN2 Protein by Mammalian Cells

      • Takashi Nishida, Satoshi Kubota, Masaharu Takigawa
      Pages 95-105

    11. In Vitro Transfection with and Expression of CCN Family of Genes

      • Danilo Janune, Masaharu Takigawa
      Pages 107-113

    12. Preparation of Module-Specific Antibodies Against CCN Family Members

      • Satoshi Kubota, Masaharu Takigawa
      Pages 115-126

    13. ELISA of CCN Family Proteins in Body Fluids Including Serum and Plasma

      • Satoshi Kubota, Harumi Kawaki, Masaharu Takigawa
      Pages 127-138

    14. Analysis of Signaling Pathways Activated by CCN Proteins

      • Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa
      Pages 139-143

    15. Protocols for Screening for Binding Partners of CCN Proteins: Yeast Two-Hybrid System

      • Mitsuhiro Hoshijima, Takako Hattori, Masaharu Takigawa
      Pages 145-154

    16. Protocols for Screening Peptide Motifs Binding to CCN Family Proteins

      • Satoshi Kubota, Harumi Kawaki, Masaharu Takigawa
      Pages 155-167

    17. Evaluation of Molecular Interaction between CCN2 Protein and Its Binding Partners by Surface Plasmon Resonance (SPR)

      • Eriko Aoyama, Masaharu Takigawa
      Pages 169-176

    18. Promoter Analyses of CCN Genes

      • Takanori Eguchi, Satoshi Kubota, Masaharu Takigawa
      Pages 177-185
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Keywords

About this book

 This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques. Chapters details practical tips to overcome any obstacles with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences and pharmacology of CCN proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, CCN Proteins: Methods and Protocols will be a valuable resource for a wide audience, ranging from the experienced CCN researchers looking for new approaches to junior graduate students just beginning in CCN research.


Editors and Affiliations

  • Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama, Japan

    Masaharu Takigawa

Bibliographic Information

  • Book Title: CCN Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Masaharu Takigawa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6430-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6428-4Published: 13 October 2016

  • Softcover ISBN: 978-1-4939-8197-7Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6430-7Published: 12 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 576

  • Number of Illustrations: 37 b/w illustrations, 67 illustrations in colour

  • Topics: Protein Science

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