Book Volume 1486 2017

Optical Tweezers

Methods and Protocols

Editors:

ISBN: 978-1-4939-6419-2 (Print) 978-1-4939-6421-5 (Online)

Table of contents (21 protocols)

previous Page of 2
  1. Front Matter

    Pages i-xii

  2. Theory and Design of Optical Tweezers

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-24

      Introduction to Optical Tweezers

    3. No Access

      Protocol

      Pages 25-39

      Exact Theory of Optical Tweezers and Its Application to Absolute Calibration

    4. No Access

      Protocol

      Pages 41-76

      Beyond the Hookean Spring Model: Direct Measurement of Optical Forces Through Light Momentum Changes

    5. No Access

      Protocol

      Pages 77-107

      A Surface-Coupled Optical Trap with 1-bp Precision via Active Stabilization

    6. No Access

      Protocol

      Pages 109-136

      Implementation and Tuning of an Optical Tweezers Force-Clamp Feedback System

    7. No Access

      Protocol

      Pages 137-155

      Custom-Made Microspheres for Optical Tweezers

    8. No Access

      Protocol

      Pages 157-181

      Optical Torque Wrench Design and Calibration

    9. No Access

      Protocol

      Pages 183-256

      High-Resolution “Fleezers”: Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection

    10. No Access

      Protocol

      Pages 257-272

      Versatile Quadruple-Trap Optical Tweezers for Dual DNA Experiments

  3. Optical Tweezers-Based Manipulation and Analysis of DNA-DNA Interactions, Protein Unfolding/Refolding, and Motion/Force-Generation by Mechanoenzymes In Vitro

    1. Front Matter

      Pages 273-273

    2. No Access

      Protocol

      Pages 275-293

      Probing DNA–DNA Interactions with a Combination of Quadruple-Trap Optical Tweezers and Microfluidics

    3. No Access

      Protocol

      Pages 295-316

      Probing Single Helicase Dynamics on Long Nucleic Acids Through Fluorescence-Force Measurement

    4. No Access

      Protocol

      Pages 317-341

      Mechanically Watching the ClpXP Proteolytic Machinery

    5. No Access

      Protocol

      Pages 343-355

      Deciphering the Molecular Mechanism of the Bacteriophage φ29 DNA Packaging Motor

    6. No Access

      Protocol

      Pages 357-390

      Single-Molecule Protein Folding Experiments Using High-Precision Optical Tweezers

    7. No Access

      Protocol

      Pages 391-409

      Observing Single RNA Polymerase Molecules Down to Base-Pair Resolution

    8. No Access

      Protocol

      Pages 411-435

      Optical Tweezers-Based Measurements of Forces and Dynamics at Microtubule Ends

    9. No Access

      Protocol

      Pages 437-467

      Simultaneous Manipulation and Super-Resolution Fluorescence Imaging of Individual Kinetochores Coupled to Microtubule Tips

    10. No Access

      Protocol

      Pages 469-481

      Measurement of Force-Dependent Release Rates of Cytoskeletal Motors

    11. No Access

      Protocol

      Pages 483-509

      Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers

  4. Quantitative Optical Tweezers Studies In Vivo

    1. Front Matter

      Pages 511-511

    2. No Access

      Protocol

      Pages 513-536

      Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap

previous Page of 2