Cell Microencapsulation

Methods and Protocols

  • Emmanuel C. Opara

Part of the Methods in Molecular Biology book series (MIMB, volume 1479)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Emmanuel C. Opara
      Pages 23-39
  3. Approaches to Cell Microencapsulation

    1. Front Matter
      Pages 41-41
    2. A. Bidoret, E. Martins, B. Poncelet De Smet, D. Poncelet
      Pages 43-55
    3. Brend Ray-Sea Hsu, Shin-Huei Fu
      Pages 57-70
    4. Varna Sharma, Michael Hunckler, Melur K. Ramasubramanian, Emmanuel C. Opara, Kalyan C. Katuri
      Pages 71-76
  4. Biomaterials and Enabling Technologies in Cell Microencapsulation

    1. Front Matter
      Pages 77-77
    2. A. Aijaz, D. Perera, Ronke M. Olabisi
      Pages 79-93
    3. William F. Kendall Jr., Emmanuel C. Opara
      Pages 95-109
    4. Marcus D. Darabbie, Emmanuel C. Opara
      Pages 111-118
    5. D. M. Lavin, B. E. Bintz, C. G. Thanos
      Pages 119-134
    6. Genaro A. Paredes-Juarez, Brad P. Barnett, Jeff W. M. Bulte
      Pages 143-155
    7. John Patrick McQuilling, Sivanandane Sittadjody, Rajesh Pareta, Samuel Pendergraft, Clancy J. Clark, Alan C. Farney et al.
      Pages 157-171
  5. Isolation of Cells for Microencapsulation

    1. Front Matter
      Pages 173-173
    2. William F. Kendall Jr., Emmanuel C. Opara
      Pages 175-189
    3. Kevin Enck, John Patrick McQuilling, Giuseppe Orlando, Riccardo Tamburrini, Sittadjody Sivanandane, Emmanuel C. Opara
      Pages 191-198
    4. J. Ciriza, L. Saenz del Burgo, R. M. Hernández, G. Orive, J. L. Pedraz
      Pages 207-216
    5. Sami I. Somo, Omaditya Khanna, Eric M. Brey
      Pages 217-224
    6. Sivanandane Sittadjody, Justin M. Saul, Emmanuel C. Opara
      Pages 225-235
    7. M. A. Sandrof, D. F. Emerich, Chris G. Thanos
      Pages 237-249
    8. Shirae K. Leslie, Ramsey C. Kinney, Zvi Schwartz, Barbara D. Boyan
      Pages 251-259
    9. L. Saenz del Burgo, J. Ciriza, R. M. Hernández, G. Orive, J. L. Pedraz
      Pages 261-272
  6. Cell Microencapsulation in Clinical Applications

    1. Front Matter
      Pages 281-281
    2. Riccardo Calafiore, Giuseppe Basta, Pia Montanucci
      Pages 283-304
    3. Rahul Krishnan, David Ko, Clarence E. Foster III, Wendy Liu, A. M. Smink, Bart de Haan et al.
      Pages 305-333
    4. Masayuki Shimoda, Shinichi Matsumoto
      Pages 335-345
    5. Masayuki Shimoda, Shinichi Matsumoto
      Pages 347-356
    6. Patricio Cabané Toledo, Ricardo L. Rossi, Pablo Caviedes
      Pages 357-363
  7. Back Matter
    Pages 365-366

About this book


This volume provides a unique forum to review cell microencapsulation in a broad sense by exploring various cell types that have been encapsulated for different purposes, different approaches and devices used for microencapsulation, the biomaterials used in cell microencapsulation, the challenges to the technology, and the current status of its application in different clinical situations. This book is divided in five sections: Section I is an introductory part that discusses historical developments of the technology and its current challenges, as well as the various applications of cell microencapsulation; Section II discusses the main approaches and devices currently used in cell microencapsulation; Section III presents an overview of the various polymeric materials currently in use for cell microencapsulation and the enabling technologies to either monitor or enhance encapsulated cell function; Section IV gives specific examples of the methods used to encapsulate various cell types; and Section V provides an overview of the different clinical situations in which cell microencapsulation has been applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Cell Microencapsulation: Methods and Protocols is a valuable reference for researchers, engineers, clinicians, and other healthcare professionals, as well as food technologists who will find detailed descriptions of methods for the microencapsulation of specific cell types and their current of potential clinical and industrial applications. This volume also includes detailed information about the design and manufacture of different devices including large-scale production devices for use in cell microencapsulation.


bioencapsulation immobilization of therapeutic cells in polymeric scaffolds semi permeable hydrogel capsules immune system microcapsules cell therapy

Editors and affiliations

  • Emmanuel C. Opara
    • 1
  1. 1.Wake Forest Institute for Regenerative Medicine Wake Forest School of MedicineWinston-SalemUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6362-1
  • Online ISBN 978-1-4939-6364-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site