Methods and Protocols

  • Manuel S. Rodriguez

Part of the Methods in Molecular Biology book series (MIMB, volume 1475)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Michael J. Matunis, Manuel S. Rodriguez
      Pages 3-22
    3. Nicole R. Wilson, Mark Hochstrasser
      Pages 23-38
  3. Procedures to Study Protein SUMOylation In Vitro

    1. Front Matter
      Pages 39-39
    2. Tobias Ritterhoff, Hrishikesh Das, Yuqing Hao, Volkan Sakin, Annette Flotho, Andreas Werner et al.
      Pages 41-54
    3. Frédérique Brockly, Marc Piechaczyk, Guillaume Bossis
      Pages 55-65
    4. Nathalie Eisenhardt, Viduth K. Chaugule, Andrea Pichler
      Pages 67-78
    5. Koraljka Husnjak, Jan Keiten-Schmitz, Stefan Müller
      Pages 79-98
    6. Valérie Lang, Elisa Da Silva-Ferrada, Rosa Barrio, James D. Sutherland, Manuel S. Rodriguez
      Pages 109-121
  4. Analysis of Protein SUMOylation Using Cell Lines

    1. Front Matter
      Pages 137-137
    2. Umut Sahin, Florence Jollivet, Caroline Berthier, Hugues de Thé, Valérie Lallemand-Breitenbach
      Pages 139-150
    3. Lucia Pirone, Wendy Xolalpa, Ugo Mayor, Rosa Barrio, James D. Sutherland
      Pages 161-169
    4. Ivo A. Hendriks, Alfred C. O. Vertegaal
      Pages 171-193
    5. Elisa Aguilar-Martínez, Andrew D. Sharrocks
      Pages 195-204
  5. Procedures to Study Protein SUMOylation In Vivo

    1. Front Matter
      Pages 217-217
    2. Ivan Psakhye, Stefan Jentsch
      Pages 219-231
    3. Federico Pelisch, Ronald T. Hay
      Pages 233-256
    4. Thérèse C. Rytz, Marcus J. Miller, Richard D. Vierstra
      Pages 257-281
    5. Katherine H. Reiter, Michael J. Matunis
      Pages 283-290
  6. Manuel S. Rodriguez
    Pages E1-E1
  7. Back Matter
    Pages 303-306

About this book


This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation. SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation of the methods included in the book. The first part also includes a review on chromatin regulation by dynamic SUMO modifications. The second part focuses on in vitro techniques, including biochemical methods to study mechanistic aspects of protein SUMOylation. The third part includes protocols to be used with cell cultures, which often are the first approaches used in most laboratories. The final part includes methodologies adapted for the analysis in vivo using distinct model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include a brief introduction to the subject, a list of necessary materials and reagents, a step-by-step reproducible laboratory protocol ending with a Notes section on troubleshooting tips, and tips and strategies to avoid known pitfalls.

Unique and cutting-edge, SUMO: Methods and Protocols provides a comprehensive source of protocols for specialists and researchers not familiar with this vital system.


small ubiquitin-related modifier SUMO enzymes protein modification SUMO molecules chromatin regulation cell cultures model organisms

Editors and affiliations

  • Manuel S. Rodriguez
    • 1
  1. 1.INBIOMEDSan SebastianSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6356-0
  • Online ISBN 978-1-4939-6358-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site