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Enhancer RNAs

Methods and Protocols

  • Ulf Andersson Ørom

Part of the Methods in Molecular Biology book series (MIMB, volume 1468)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Thomas Conrad, Ulf Andersson Ørom
    Pages 1-9
  3. Benoit T. Roux, Mark A. Lindsay, James A. Heward
    Pages 11-18
  4. Youtaro Shibayama, Stephanie Fanucchi, Musa M. Mhlanga
    Pages 19-32
  5. Katie Schaukowitch, Jae-Yeol Joo, Tae-Kyung Kim
    Pages 33-38
  6. Steven Blinka, Michael H. Reimer Jr., Kirthi Pulakanti, Luca Pinello, Guo-Cheng Yuan, Sridhar Rao
    Pages 91-109
  7. Alessandro Gardini
    Pages 111-120
  8. Anusha Nagari, Shino Murakami, Venkat S. Malladi, W. Lee Kraus
    Pages 121-138
  9. Ana Carolina Ayupe, Eduardo M. Reis
    Pages 139-153
  10. C. Zurla, J. Jung, E. L. Blanchard, P. J. Santangelo
    Pages 155-170
  11. Xinyi Yang, Annalisa Marsico
    Pages 171-199
  12. Sarah Djebali, Valentin Wucher, Sylvain Foissac, Christophe Hitte, Erwan Corre, Thomas Derrien
    Pages 201-219
  13. Guillaume Andrey, Malte Spielmann
    Pages 221-234
  14. Alexander Brown, Wendy S. Woods, Pablo Perez-Pinera
    Pages 235-250
  15. Sarah Djebali, Valentin Wucher, Sylvain Foissac, Christophe Hitte, Erwan Corre, Thomas Derrien
    Pages E1-E1
  16. Back Matter
    Pages 251-252

About this book

Introduction

This volume provides a comprehensive overview of the experimental and computational methodologies used to study the function of long non-coding RNA (ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and exploring transcription by assessing where transcription starts and generally how it occurs.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Enhancer RNAs: Methods and Protocols aims to ensure successful results in this rapidly developing field.

Keywords

long ncRNAs transcription genome-wide RNA manipulation siRNA antisense oligos

Editors and affiliations

  • Ulf Andersson Ørom
    • 1
  1. 1.Max Planck Institute Molecular GeneticsBerlinGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-4035-6
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-4033-2
  • Online ISBN 978-1-4939-4035-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site