Methods and Protocols

  • Sung Bae Kim

Part of the Methods in Molecular Biology book series (MIMB, volume 1461)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Ingredients of Bioluminescent Probes

  3. Fabrication of Bioluminescent Probes

    1. Front Matter
      Pages 63-63
    2. Wenxiao Wu, Laizhong Chen, Jing Li, Lupei Du, Minyong Li
      Pages 91-99
    3. Yunfei Zhang, J. Brian Robertson, Qiguang Xie, Carl Hirschie Johnson
      Pages 117-130
    4. Sung-Bae Kim, Hiroaki Tao
      Pages 143-151
    5. Sung-Bae Kim, Yoshio Umezawa
      Pages 153-163
    6. Thillai V. Sekar, Ramasamy Paulmurugan
      Pages 203-215
  4. Applications to Living Subjects and Instrumentations

    1. Front Matter
      Pages 225-225
    2. Joshua Bakhsheshian, Bih-Rong Wei, Matthew D. Hall, R. Mark Simpson, Michael M. Gottesman
      Pages 227-239
    3. Thillai V. Sekar, Ramasamy Paulmurugan
      Pages 241-254
    4. Vicky T. Knol-Blankevoort, Laura Mezzanotte, Martijn J. W. E. Rabelink, Clemens W. G. M. Löwik, Eric L. Kaijzel
      Pages 255-270
    5. Sung-Bae Kim, Ryuichi Naganawa
      Pages 271-278
    6. Sung-Bae Kim, Ryuichi Naganawa, Shingo Murata, Takayoshi Nakayama, Simon Miller, Toshiya Senda
      Pages 279-287
  5. Back Matter
    Pages 311-314

About this book


This volume presents detailed laboratory protocols regarding the three major route technologies luciferases, efficient optical probes, and applications to visualizing molecular events in living subjects. Bioluminescence: Methods and Protocols, Third Edition guides readers through chapters on ingredients of bioluminescent probes, and fabrication of bioluminescent probes, applications to living subjects and instrumentations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Bioluminescence: Methods and Protocols, Third Edition is a useful complement to the first and second edition for new and experienced researchers alike.




luciferase optical probes molecular events de novo luciferases optical performance

Editors and affiliations

  • Sung Bae Kim
    • 1
  1. 1.Natl Inst of AIST Res Inst for Envt Mgt TechTsukubaJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3811-7
  • Online ISBN 978-1-4939-3813-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site