Unconventional Protein Secretion

Methods and Protocols

  • Andrea Pompa
  • Francesca De Marchis

Part of the Methods in Molecular Biology book series (MIMB, volume 1459)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Background

    1. Front Matter
      Pages 1-1
    2. Fanny Ng, Bor Luen Tang
      Pages 31-46
    3. Destiny J. Davis, Byung-Ho Kang, Angelo S. Heringer, Thomas E. Wilkop, Georgia Drakakaki
      Pages 47-63
  3. UPS Contemplates Multidisciplinary Approaches for Plants

  4. UPS Contemplates Multidisciplinary Approaches for Animals and Fungi

    1. Front Matter
      Pages 103-103
    2. Tania Arcondéguy, Christian Touriol, Eric Lacazette
      Pages 127-134
    3. Gerhard E. Strittmatter, Martha Garstkiewicz, Jennifer Sand, Serena Grossi, Hans-Dietmar Beer
      Pages 135-147
    4. Marcos Vinicios Salles Dias, Vilma Regina Martins, Glaucia Noeli Maroso Hajj
      Pages 161-174
    5. Marcio L. Rodrigues, Debora L. Oliveira, Gabriele Vargas, Wendell Girard-Dias, Anderson J. Franzen, Susana Frasés et al.
      Pages 175-190
  5. Secretome Isolation from Plant and Animal Samples to Identify Leaderless Secretory Proteins (LSP)

    1. Front Matter
      Pages 229-229
    2. Bertrand Delaunois, Fabienne Baillieul, Christophe Clément, Philippe Jeandet, Sylvain Cordelier
      Pages 249-257
    3. Christopher Stanly, Immacolata Fiume, Giovambattista Capasso, Gabriella Pocsfalvi
      Pages 259-269
  6. Back Matter
    Pages 271-273

About this book


This volume presents relevant background information to understanding the molecular basis governing unconventional protein secretion (UPS), and in particular explores the latest techniques and protocols that have been successfully applied for the study of this topic. Detailed chapters include an overview of conventional and unconventional secretory pathways along with multidisciplinary approaches and methods used for UPS analysis in different organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Unconventional Protein Secretion: Methods and Protocols will be useful for all interested in the secretory pathway field as well as applications in cell biology, cell development, biomedical research, and healthcare.


secretory pathways UPS analysis organisms SP lacking proteins protein traffic

Editors and affiliations

  • Andrea Pompa
    • 1
  • Francesca De Marchis
    • 2
  1. 1.Instituto di Bioscienze e BiorisorsePerugiaItaly
  2. 2.Instituto di Bioscienze e BiorisorsePerugiaItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3802-5
  • Online ISBN 978-1-4939-3804-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site