The Tumor Microenvironment

Methods and Protocols

  • Josie Ursini-Siegel
  • Nicole Beauchemin

Part of the Methods in Molecular Biology book series (MIMB, volume 1458)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Nicholas R. Bertos, Morag Park
    Pages 13-25
  3. Lian Willetts, David Bond, Konstantin Stoletov, John D. Lewis
    Pages 27-37
  4. Esterina D’Asti, Brian Meehan, Janusz Rak
    Pages 39-58
  5. José A. Jiménez-Torres, David J. Beebe, Kyung E. Sung
    Pages 59-69
  6. Eelco F. J. Meijer, James W. Baish, Timothy P. Padera, Dai Fukumura
    Pages 71-85
  7. Yoon Kow Young, Alicia M. Bolt, Ryuhjin Ahn, Koren K. Mann
    Pages 95-110
  8. Kelly A. Pike, Caitlyn Hui, Connie M. Krawczyk
    Pages 111-124
  9. Joanne A. Hammill, Arya Afsahi, Jonathan L. Bramson, Christopher W. Helsen
    Pages 137-157
  10. Jitka Y. Sagiv, Sandra Voels, Zvi Granot
    Pages 179-193
  11. Khalid Al-Nedawi, Jolene Read
    Pages 195-202
  12. Victor Naumenko, Craig Jenne, Douglas J. Mahoney
    Pages 217-230
  13. Chirayu Chokshi, Manvir Dhillon, Nicole McFarlane, Chitra Venugopal, Sheila K. Singh
    Pages 231-245
  14. Simon-Pierre Gravel, Daina Avizonis, Julie St-Pierre
    Pages 273-290
  15. Pudchalaluck Panichnantakul, Mathieu Bourgey, Alexandre Montpetit, Guillaume Bourque, Yasser Riazalhosseini
    Pages 311-337
  16. Alice Beigbeder, Lauriane Vélot, D. Andrew James, Nicolas Bisson
    Pages 339-347
  17. Back Matter
    Pages 349-356

About this book


This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis. In addition, the book examines research topics including cellular and molecular biology approaches, in vivo genetic approaches, various “omics”-based strategies, therapeutic strategies to target the microenvironment, and, finally, advanced techniques in the fields of tissue engineering and nanotechnology.  Written and validated in the laboratories of a number of trusted collaborating authors for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Practical and authoritative, The Tumor Microenvironment: Methods and Protocols constitutes a compendium of techniques now available to a broad audience, including basic and clinician scientists, systems biologists, and biological engineers.


Tumor cells Carcinogenesis Therapeutic strategies Genetic approaches Cancer research Extracellular matrix

Editors and affiliations

  • Josie Ursini-Siegel
    • 1
  • Nicole Beauchemin
    • 2
  1. 1.Lady Davis Institute for Medical ResearchMontrealCanada
  2. 2.Goodman Cancer Research CentreMcGill UniversityMontrealCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3799-8
  • Online ISBN 978-1-4939-3801-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site