Non-Viral Gene Delivery Vectors

Methods and Protocols

  • Gabriele Candiani

Part of the Methods in Molecular Biology book series (MIMB, volume 1445)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Conventional Bolus Gene Delivery Vectors

    1. Front Matter
      Pages 1-1
    2. Emile Jubeli, William P. D. Goldring, Michael D. Pungente
      Pages 19-32
    3. Ana L. Barrán-Berdón, Emilio Aicart, Elena Junquera
      Pages 45-61
    4. Edilberto Ojeda, Mireia Agirre, Ilia Villate-Beitia, Mohamed Mashal, Gustavo Puras, Jon Zarate et al.
      Pages 63-75
    5. Margarida I. Simão Carlos, Andreas Schätzlein, Ijeoma Uchegbu
      Pages 149-157
    6. Magdalena Wytrwal, Chantal Pichon
      Pages 159-174
    7. Daniel G. Abebe, Rima Kandil, Teresa Kraus, Maha Elsayed, Tomoko Fujiwara, Olivia M. Merkel
      Pages 175-185
    8. Xiaomeng Huang, Mengzi Zhang, Xinmei Wang, L. James Lee, Robert J. Lee
      Pages 201-213
  3. Stimuli-Responsive Bolus Gene Delivery Vectors

    1. Front Matter
      Pages 215-215
    2. Daniele Pezzoli, Elena Tallarita, Elena Rosini, Gabriele Candiani
      Pages 217-233
    3. Stephan Morys, Ernst Wagner, Ulrich Lächelt
      Pages 235-258
    4. Nan Zheng, Yang Liu, Jianjun Cheng
      Pages 259-267
  4. Substrate-Mediated Gene Delivery

    1. Front Matter
      Pages 277-277
    2. Hongwei Wang, Jingjing Pan, Hong Chen, Lin Yuan
      Pages 279-287
  5. Back Matter
    Pages 289-290

About this book


This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuli-responsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substrate-mediated gene delivery—also termed reverse transfection—and the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field.


conventional bolus gene delivery vectors cell culture medium stimuli-responsive bolus transfectants polymers reverse transfection solid-phase delivery cationic lipid-based lipoplexes zwitterionic lipid-based lipoplexes polymer-strenthened lipid-coated

Editors and affiliations

  • Gabriele Candiani
    • 1
  1. 1.Polytechnic University of MilanMilanItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3716-5
  • Online ISBN 978-1-4939-3718-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site