West Nile Virus

Methods and Protocols

  • Tonya M. Colpitts

Part of the Methods in Molecular Biology book series (MIMB, volume 1435)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Berlin Londono-Renteria, Tonya M. Colpitts
    Pages 1-13
  3. Andrea Troupin, Tonya M. Colpitts
    Pages 15-18
  4. Alexander J. McAuley, David W. C. Beasley
    Pages 19-27
  5. Rianna Vandergaast, Brenda L. Fredericksen
    Pages 29-43
  6. Pakieli H. Kaufusi, Alanna Tseng, Vivek R. Nerurkar
    Pages 45-60
  7. Brenna McGruder, Vandana Saxena, Tian Wang
    Pages 61-69
  8. Brian M. Friedrich, David W. C. Beasley
    Pages 129-141
  9. Dexter R. McKellar, Michael J. Conway
    Pages 143-150
  10. Samuel B. Jameson, Dawn M. Wesson
    Pages 165-174
  11. Elizabeth B. Kauffman, Mary A. Franke, Laura D. Kramer
    Pages 175-206
  12. Sarah S. Wheeler, Walter M. Boyce, William K. Reisen
    Pages 207-220
  13. Yi Yao, Ruth R. Montgomery
    Pages 235-247
  14. Back Matter
    Pages 293-295

About this book


This volume is a collection of research methods, techniques, and approaches to investigate the molecular biology of West Nile Virus (WNV). Chapters in the book cover many facets of WNV, such as propagation and titration of WNV in vero cells, examination of WNV neuroinvasion and neuropathogenesis in the central nervous system of a murine model, field surveillance methods for WNV, and in vitro and in vivo blood-brain barrier models to study WNV pathogenesis. A brief introduction, along with a discussion on WNV laboratory safety are also included. Written in the highly successful Methods in Molecular Biology series format, chapters includes introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, West Nile Virus will interest virologists, molecular biologists, microbiologists, and other scientists working with WNV. This book is a comprehensive guide for those conducting research in the field of WNV biology, including life cycles, pathogenesis, impact of infection, and possible treatment/prevention development.


West Nile Virus infection infectious clone field surveillance immune aging transmission dynamics detection protocols neuroinvasion neuropathogenesis

Editors and affiliations

  • Tonya M. Colpitts
    • 1
  1. 1.Dept. of Path., MicroBio., and Immun.Univ. of South Carolina School of Med.ColumbiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3668-7
  • Online ISBN 978-1-4939-3670-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site