Angiogenesis Protocols

  • Stewart G. Martin
  • Peter W. Hewett

Part of the Methods in Molecular Biology book series (MIMB, volume 1430)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Microscopic Assessment

    1. Front Matter
      Pages 1-1
    2. Jia-Min Pang, Nicholas Jene, Stephen B. Fox
      Pages 3-33
    3. Daniel J. Royston, Steven Clasper, David G. Jackson
      Pages 35-48
    4. Narmeen S. Ahmad, Stewart G. Martin, Sarah J. Storr
      Pages 49-57
  3. Cell Isolation Techniques

    1. Front Matter
      Pages 59-59
    2. Jessal J. Patel, Salil Srivastava, Richard C. M. Siow
      Pages 91-105
    3. Vincent A. Primo, Joseph F. Arboleda-Velasquez
      Pages 107-117
  4. In Vitro Techniques

    1. Front Matter
      Pages 119-119
    2. Ryan M. Brown, Christopher J. Meah, Victoria L. Heath, Iain B. Styles, Roy Bicknell
      Pages 149-157
    3. Mark Richards, Harry Mellor
      Pages 159-166
    4. Larissa Pfisterer, Thomas Korff
      Pages 167-177
    5. Giorgio Seano, Luca Primo
      Pages 191-203
    6. Nina Zippel, Yindi Ding, Ingrid Fleming
      Pages 205-219
    7. Lynn M. Butler, Helen M. McGettrick, Gerard B. Nash
      Pages 231-248
  5. In Vivo Techniques

    1. Front Matter
      Pages 249-249
    2. Gillian M. Tozer, Vivien E. Prise, Vincent J. Cunningham
      Pages 265-281
    3. Lucia Morbidelli, Marina Ziche
      Pages 299-310
    4. Shintaro Nakao, Ali Hafezi-Moghadam
      Pages 311-316
    5. Melissa V. Gammons, David O. Bates
      Pages 317-332
    6. Silvia Passos Andrade, Mônica Alves Neves Diniz Ferreira
      Pages 333-343
    7. Stuart Egginton
      Pages 355-373
    8. Emma Ristori, Sandra Donnini, Marina Ziche
      Pages 387-400
  6. Back Matter
    Pages 401-404

About this book


In addition to updating important core techniques this third edition presents new chapters on assessing leukocyte involvement in angiogenesis, lymphatic cell and pericyte isolation techniques, spheroid and arterial ring based in vitro assays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols, Third Edition also guides readers through new protocols on chorioallantoic membrane models, corneal pocket assays to assess angio- and lymphangiogenesis, models of muscle angiogenesis, and use of zebrafish embryos to study vascular angiogenesis and senescence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Angiogenesis Protocols, Third Edition provides not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.


assessing leukocyte lymphatic cell pericyte isolation techniques angiogenesis chorioallantoic membrane models models of muscle angiogenesis zebrafish embryos

Editors and affiliations

  • Stewart G. Martin
    • 1
  • Peter W. Hewett
    • 2
  1. 1.NottinghamUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3626-7
  • Online ISBN 978-1-4939-3628-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site