Synthetic mRNA

Production, Introduction Into Cells, and Physiological Consequences

  • Robert E. Rhoads

Part of the Methods in Molecular Biology book series (MIMB, volume 1428)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Introduction

  3. Synthesis of mRNA

    1. Front Matter
      Pages 29-29
    2. Joanna Kowalska, Franck Martin, Jacek Jemielity
      Pages 31-43
    3. Josephin M. Holstein, Daniela Stummer, Andrea Rentmeister
      Pages 45-60
    4. Artem V. Domashevskiy, David J. Rodriguez, Dilantha Gunawardana, Dixie J. Goss
      Pages 61-75
    5. Wei Su, Michael K. Slevin, William F. Marzluff, Robert E. Rhoads
      Pages 93-114
    6. Daphné Benteyn, Carlo Heirman, Kris Thielemans, Aude Bonehill
      Pages 115-123
  4. Introduction of Synthetic mRNA into Cells

    1. Front Matter
      Pages 125-125
    2. Christina L. Gardner, Derek W. Trobaugh, Kate D. Ryman, William B. Klimstra
      Pages 127-137
    3. Judith Derdelinckx, Zwi N. Berneman, Nathalie Cools
      Pages 139-150
    4. David Selmeczi, Thomas Steen Hansen, Özcan Met, Inge Marie Svane, Niels B. Larsen
      Pages 151-161
    5. Sebastian Kreiter, Mustafa Diken, Abderraouf Selmi, Jutta Petschenka, Özlem Türeci, Ugur Sahin
      Pages 163-175
    6. Jens Dannull, Smita K. Nair
      Pages 177-186
    7. Gábor Boros, Katalin Karikó, Hiromi Muramatsu, Edit Miko, Eszter Emri, Csaba Hegedűs et al.
      Pages 219-228
  5. Alteration of Cellular Function with Synthetic mRNA

    1. Front Matter
      Pages 229-229
    2. Troels Holz Borch, Inge Marie Svane, Özcan Met
      Pages 245-259
    3. Manja Idorn, Per thor Straten, Inge Marie Svane, Özcan Met
      Pages 261-276
    4. An Coosemans, Sandra Tuyaerts, Kim Morias, Jurgen Corthals, Carlo Heirman, Kris Thielemans et al.
      Pages 277-283
    5. Sarene Koh, Noriko Shimasaki, Antonio Bertoletti
      Pages 285-296
    6. Song Lu, Christie C. Chow, Junwei Zhou, Po Sing Leung, Stephen K. Tsui, Kathy O. Lui
      Pages 307-317
  6. Back Matter
    Pages 319-320

About this book


This volume presents detailed laboratory protocols for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo-chemical crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced activation of the innate immune response. Protocols are described for clinical applications such as large-scale transfection of dendritic cells, production of GMP-grade mRNA, redirecting T cell specificity, and use of molecular adjuvants for RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences
is a valuable and cutting-edge resource for both laboratory investigators and clinicians interested in this powerful and rapidly evolving technology. 


gene therapy DNA lineage conversion of cell fates genome editing mRNA protein replacement therapy Immunotherapy cancer and infectious diseases pluripotent stem cells somatic cells

Editors and affiliations

  • Robert E. Rhoads
    • 1
  1. 1.Dept of Biochemistry&Molecular BioLouisiana State Univ Health Scien centerShreveportUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3623-6
  • Online ISBN 978-1-4939-3625-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site