© 2016

Dendritic Cell Protocols

  • Elodie Segura
  • Nobuyuki Onai

Part of the Methods in Molecular Biology book series (MIMB, volume 1423)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. In Vitro Culture of Dendritic Cells

    1. Front Matter
      Pages 17-17
    2. Matteo Pigni, Devika Ashok, Hans Acha-Orbea
      Pages 39-49
  4. Purification and Isolation of Dendritic Cell Subsets

    1. Front Matter
      Pages 51-51
    2. Charlotte L. Scott, Pamela B. Wright, Simon W. F. Milling, Allan McI Mowat
      Pages 101-118
    3. Merry Gunawan, Laura Jardine, Muzlifah Haniffa
      Pages 119-128
    4. Camille Malosse, Sandrine Henri
      Pages 129-137
    5. Lianne van de Laar, Martin Guilliams, Simon Tavernier
      Pages 139-152
    6. Solana Alculumbre, Lucia Pattarini
      Pages 153-167
    7. Naomi McGovern, Andreas Schlitzer, Baptiste Janela, Florent Ginhoux
      Pages 169-180
  5. Ex Vivo Functional Analysis of Dendritic Cells

    1. Front Matter
      Pages 181-181
    2. Omar I. Vivar, Joao G. Magalhaes, Sebastian Amigorena
      Pages 183-188
    3. Haiyin Liu, Claire Dumont, Angus P. R. Johnston, Justine D. Mintern
      Pages 199-209

About this book


The third edition of this volume is aimed at providing both beginners and more experienced researchers a choice of methods to isolate and analyze dendritic cells(DC). An introductory review provides an overview of recent advances in the characterization of DC subsets in mouse and human. While additional chapters provide methods to culture human and mouse dendritic cells, protocols for the isolation of dendritic cells, the isolation of dendritic cell progenitors from mouse, and the purification of dendritic cells from human blood. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge,  Dendritic Cell Protocols, Third Edition aims to ensure successful results in the further study of this vital field.


culture human dendritic cells culture mouse dendritic cells dendritic cell progenitors human blood DC subsets

Editors and affiliations

  • Elodie Segura
    • 1
  • Nobuyuki Onai
    • 2
  1. 1.Pavillon PasteurInstitut Curie INSERM U932Paris Cedex 05France
  2. 2.Department of BiodefenseTokyo Medical and Dental UniversityTokyoJapan

Bibliographic information

  • Book Title Dendritic Cell Protocols
  • Editors Elodie Segura
    Nobuyuki Onai
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-3604-5
  • Softcover ISBN 978-1-4939-8092-5
  • eBook ISBN 978-1-4939-3606-9
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 3
  • Number of Pages XI, 322
  • Number of Illustrations 41 b/w illustrations, 27 illustrations in colour
  • Topics Immunology
  • Buy this book on publisher's site