Forensic DNA Typing Protocols

  • William Goodwin

Part of the Methods in Molecular Biology book series (MIMB, volume 1420)

Table of contents

  1. Front Matter
    Pages i-x
  2. Roland A. H. van Oorschot, Timothy J. Verdon, Kaye N. Ballantyne
    Pages 1-12
  3. Amy D. Roeder, Cordula Haas
    Pages 13-31
  4. Joanna Jakubowska, Agnieszka Maciejewska, Ryszard Pawłowski
    Pages 33-42
  5. Wafa Altayari
    Pages 55-68
  6. Suni M. Edson, Timothy P. McMahon
    Pages 69-87
  7. Irena Zupanič Pajnič
    Pages 89-108
  8. Majid Bashir, Nur Haliza Bt Hassan
    Pages 135-142
  9. Igor V. Ovchinnikov, Mathew J. Malek, Katelyn Kjelland, Kenneth Drees
    Pages 157-171
  10. B. Bekaert, R. Ellerington, L. Van den Abbeele, R. Decorte
    Pages 173-183
  11. Chris Phillips, Carla Santos, Manuel Fondevila, Ángel Carracedo, Maria Victoria Lareu
    Pages 233-253
  12. Carla Santos, Chris Phillips, A. Gomez-Tato, J. Alvarez-Dios, Ángel Carracedo, Maria Victoria Lareu
    Pages 255-285

About this book

Introduction

This volume presents a series of protocols and methods, some of which are not widely used by researchers/practitioners, and will aid in the execution of different laboratory techniques. Forensic DNA Typing Protocols, Second Edition is arranged into a series of related chapters. Chapter 1-3 examines two different aspects of RNA analysis for body fluid identification. Chapters 4-7 focuses on the storage of biological materials and the extraction of DNA from hard tissues. Chapters 8-10 present methods for monitoring the quality of DNA extracts, and steps to aid in the purification of DNA. Chapters 11-16 talk about methods on non-standard markers, such as INDELs, Y chromosome STRs, and mitochondrial DNA. Detailed procedures and data analysis for phenotypes and ancestry are explored in Chapter 17-19. The last chapter (20) looks at the application of DNA typing to the identification of non-human material to species level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Forensic DNA Typing Protocols, Second Edition, is a valuable resource for forensic specialists, researchers, and anyone interested in the field of forensic science.

 

Keywords

single nucleotide polymorphisms (SNPS) insertion-deletion polymorphisms (INDELs) Next Generation Sequencing (NGS) technology DNA profiling RNA analysis DNA extraction Y chromosome STRs mitochondrial DNA

Editors and affiliations

  • William Goodwin
    • 1
  1. 1.School of Forensic and Applied SciencesUniversity of Central LancashirePrestonUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3597-0
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3595-6
  • Online ISBN 978-1-4939-3597-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book