Programmed Cell Death

Methods and Protocols

  • Hamsa Puthalakath
  • Christine J. Hawkins

Part of the Methods in Molecular Biology book series (MIMB, volume 1419)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Valentina Sica, M. Chiara Maiuri, Guido Kroemer, Lorenzo Galluzzi
    Pages 1-16
  3. Laura E. Edgington-Mitchell, Matthew Bogyo
    Pages 27-39
  4. Thomas Grabinger, Eugenia Delgado, Thomas Brunner
    Pages 83-93
  5. Ramona Reinhart, Simone Wicki, Thomas Kaufmann
    Pages 95-107
  6. George Williams Mbogo, Christina Nedeva, Hamsa Puthalakath
    Pages 117-129
  7. Deepika Vasudevan, Hyung Don Ryoo
    Pages 131-144
  8. Joanna Kacprzyk, Adrian N. Dauphinee, Patrick Gallois, Arunika HLAN Gunawardena, Paul F. McCabe
    Pages 145-160
  9. David T. Bloomer, Tanja Kitevska, Ingo L. Brand, Anissa M. Jabbour, Hang Nguyen, Christine J. Hawkins
    Pages 161-183
  10. Marc Kvansakul, Peter E. Czabotar
    Pages 213-229
  11. Boris Reljić, David A. Stroud
    Pages 231-252
  12. Andrew J. Kueh, Marco J. Herold
    Pages 253-264
  13. Ueli Nachbur, Gabriela Brumatti
    Pages 265-276

About this book

Introduction

This volume contains protocols specifically designed for studying programmed cell death, and also discusses recent advances in techniques that span broader areas of biology that have been recently used or that have potential to be incorporated into cell death research. The protocols are mostly described in the context of mammalian systems, but also cover other systems such as plants, Drosophila, and yeast. Programmed Cell Death: Methods and Protocols is comprised of 20 chapters: Chapters 1-5 describe apoptosis detection techniques; Chapter 6-9 describe methods for studying apoptosis associated with various pathologies in different organs including the lymphoid compartment, intestinal epithelium, granulocytes, and cardiomyocytes; Chapter 11-13 cover protocols and techniques for studying apoptosis in non-mammalian systems; Chapters 14-16 cover biochemical and biophysical methods for studying Bcl-2 family protein dynamics and protein-protein interactions during apoptosis; and the last four chapters explore protocols that are useful not only in apoptosis research but in wider areas of biological research, such as genome editing, inducible transgenes, and proteomics. Written in the highly successful Methods in Molecular Biology aeries format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocol, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Programmed Cell Death: Methods and Protocols is a comprehensive and valuable resource for researchers, ranging from beginner to expert, in their studies on programmed cell death.

 

Keywords

delineating the molecular mechanisms Drosophila metazoan apoptosis process apoptosis detection techniques autophagic cell death processes caspase activity initiation at single cell level peptide-based techniques for detecting caspases Bcl-2 family protein dynamics protein-protein interactions genome editing apoptosis

Editors and affiliations

  • Hamsa Puthalakath
    • 1
  • Christine J. Hawkins
    • 2
  1. 1.Department of Biochemistry and GeneticsLa Trobe Inst for Mol Sci, La Trobe UnivBundooraAustralia
  2. 2.Department of Biochemistry and GeneticsLa Trobe Inst for Mol Sci, La Trobe UnivBundooraAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3581-9
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3579-6
  • Online ISBN 978-1-4939-3581-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book