Animal Coronaviruses

  • Leyi Wang

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Leyi Wang, Yan Zhang
      Pages 3-11
  3. Conventional Technologies

  4. Reverse Genetics

    1. Front Matter
      Pages 51-51
    2. Sarah M. Keep, Erica Bickerton, Paul Britton
      Pages 53-72
  5. Molecular Diagnostics

    1. Front Matter
      Pages 91-91
    2. Fabien Miszczak, Nathalie Kin, Vincent Tesson, Astrid Vabret
      Pages 93-100
    3. Amauri Alcindo Alfieri, Alice Fernandes Alfieri, Elisabete Takiuchi
      Pages 101-113
    4. Yi-Ning Chen, Aydemir Akin, Chien Chang Loa, Mustafa Ababneh, Jianzhong Cao, Wan-Jung Chen et al.
      Pages 151-160
    5. Rita de Cássia Nasser Cubel Garcia
      Pages 189-198
  6. Next Generation Sequencing

    1. Front Matter
      Pages 199-199
    2. Leyi Wang, Tod Stuber, Patrick Camp, Suelee Robbe-Austerman, Yan Zhang
      Pages 201-208
    3. Douglas Marthaler, Ann Bohac, Aaron Becker, Nichole Peterson
      Pages 209-216
  7. Back Matter
    Pages 217-218

About this book


This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.


Coronaviridae Reverse genetics Molecular diagnostics Next generation sequencing Virology Immunohistochemistry

Editors and affiliations

  • Leyi Wang
    • 1
  1. 1.Animal Disease Diagnostic LabOhio Department of AgricultureReynoldsburgUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3412-6
  • Online ISBN 978-1-4939-3414-0
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site