Toll-Like Receptors

Practice and Methods

  • Claire E. McCoy

Part of the Methods in Molecular Biology book series (MIMB, volume 1390)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Toll-Like Receptor Detection and Activation

    1. Front Matter
      Pages 1-1
    2. Jennifer K. Dowling, Jérome Dellacasagrande
      Pages 3-27
    3. Tom P. Monie, Nicholas J. Gay, Monique Gangloff
      Pages 29-39
    4. Gabor L. Horvath, Pia Langhoff, Eicke Latz
      Pages 41-64
    5. Jonathan Ferrand, Michael P. Gantier
      Pages 79-90
  3. Toll-Like Receptor Cross-Priming of Associated Receptors

    1. Front Matter
      Pages 91-91
    2. Katryn J. Stacey, Adi Idris, Vitaliya Sagulenko, Nazarii Vitak, David P. Sester
      Pages 93-106
    3. Joris K. Sprokholt, Nina Hertoghs, Teunis B. H. Geijtenbeek
      Pages 121-129
  4. Toll-Like Receptor Post-Transcriptional Regulation

    1. Front Matter
      Pages 143-143
    2. Timothy Ravasi, Charalampos (Harris) Mavromatis, Nilesh J. Bokil, Mark A. Schembri, Matthew J. Sweet
      Pages 145-158
  5. Toll-Like Receptors and System Control

    1. Front Matter
      Pages 213-213
    2. Stephanie Flaherty, Joseph M. Reynolds
      Pages 215-227
    3. Krishna L. Moody, Melissa B. Uccellini, Ana M. Avalos, Ann Marshak-Rothstein, Gregory A. Viglianti
      Pages 249-272
    4. Daniel G. W. Johnston, Sinéad C. Corr
      Pages 287-300
  6. Toll-Like Receptors and Disease

    1. Front Matter
      Pages 317-317
    2. Marc Schmidt, Matthias Goebeler, Stefan F. Martin
      Pages 319-340
    3. Tom P. Monie, Clare E. Bryant
      Pages 341-350
    4. Anna M. Piccinini, Lynn Williams, Fiona E. McCann, Kim S. Midwood
      Pages 351-381
    5. Francesca Fallarino, Marco Gargaro, Giada Mondanell, Eric J. Downer, Md Jakir Hossain, Bruno Gran
      Pages 383-411
    6. Lucien P. Garo, Gopal Murugaiyan
      Pages 413-425
  7. Back Matter
    Pages 451-455

About this book


This second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction with other cellular and biologic systems, and cancer progression. This book is divided into five sections: Part I outlines methods for TLR detection, interaction, and intracellular trafficking; Part II describes methods and assays to investigate how TLRs cross-prime other pattern recognition receptors, including intracellular DNA receptors and inflammasome formation; Part III highlights RNA regulation, detailing how TLRs can induce RNA transcripts and molecules such as lncRNAs and microRNAs; Part IV explores TLR detection and activation in systems such as epithelial barrier function, metabolism and the circadian clock, as well as cellular systems including T and B lymphocytes; and Part V describes models to delineate the role of TLRs in diseases such as dermatitis, arthritis, and gastric cancer. Written in the highly successful Methods in Molecular Biology series format, each chapter contains a summary, a list of required materials, step-by-step, readily reproducible laboratory protocols, useful notes to investigate TLRs in cell culture, systems and disease, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Toll-Like Receptors: Methods and Protocols, Second Edition is a valuable resource to any immunologist, molecular or medical biologist working in a laboratory setting. It will add skill to both students and the more advanced molecular biologist who wishes to learn a new technique or move to a different area within their current repertoire of practical knowledge.


Toll-Like Receptors (TLRs) cross-priming of associated pattern recognition receptors cancer progression intracellular DNA receptors C-type lectin receptors transmembrane proteins microRNAs long noncoding RNAs immune response T and B lymphocytes

Editors and affiliations

  • Claire E. McCoy
    • 1
  1. 1.Hudson Institute of Medical ResearchMonash University Faculty of MedicineClaytonAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3333-4
  • Online ISBN 978-1-4939-3335-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site