Imaging Flow Cytometry

Methods and Protocols

  • Natasha S. Barteneva
  • Ivan A. Vorobjev

Part of the Methods in Molecular Biology book series (MIMB, volume 1389)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. General

    1. Front Matter
      Pages 1-1
    2. Ivan A. Vorobjev, Natasha S. Barteneva
      Pages 3-11
    3. David A. Basiji
      Pages 13-21
    4. Andy K. S. Lau, Terence T. W. Wong, Ho Cheung Shum, Kenneth K. Y. Wong, Kevin K. Tsia
      Pages 23-45
    5. Mark Hildebrand, Aubrey Davis, Raffaela Abbriano, Haley R. Pugsley, Jesse C. Traller, Sarah R. Smith et al.
      Pages 47-67
  3. IFC in Detection of Nuclei and Nucleic Acids

    1. Front Matter
      Pages 69-69
    2. Andrew Filby, William Day, Sukhveer Purewal, Nuria Martinez-Martin
      Pages 71-95
    3. Lindsay A. Beaton-Green, Ruth C. Wilkins
      Pages 97-110
    4. Orla Maguire, Paul K. Wallace, Hans Minderman
      Pages 111-126
    5. Elizaveta Fasler-Kan, Yeldar Baiken, Ivan A. Vorobjev, Natasha S. Barteneva
      Pages 127-137
  4. IFC in Immune Response and Drug Screening

    1. Front Matter
      Pages 153-153
    2. Ana M. Dias, Catarina R. Almeida, Celso A. Reis, Salomé S. Pinho
      Pages 167-176
    3. Brian K. McFarlin, Adam S. Venable, Andrea L. Henning, Randall R. Williams, Eric A. Prado
      Pages 177-185
    4. Andrea L. Henning, Adam S. Venable, Eric A. Prado, Brian K. McFarlin
      Pages 187-194
  5. IFC in Studies of Cell Death and Apoptosis

    1. Front Matter
      Pages 207-207
    2. Aja M. Rieger, Daniel R. Barreda
      Pages 209-220
    3. Ivan A. Vorobjev, Natasha S. Barteneva
      Pages 221-233
  6. Imaging Cytometry in Cell Classification and Morphological Analysis

    1. Front Matter
      Pages 235-235
    2. Lisa M. Niswander, James Palis, Kathleen E. McGrath
      Pages 265-277
    3. Kleber Y. Fertrin, Leigh Samsel, Eduard J. van Beers, Laurel Mendelsohn, Gregory J. Kato, J. Philip McCoy Jr.
      Pages 279-292
  7. Back Matter
    Pages 293-295

About this book


This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.


Apoptosis Cell death analysis Drug screening IDEAS software IFC Immune response analysis Morphological analysis Nucleic acids Quantitative imaging flow cytometry

Editors and affiliations

  • Natasha S. Barteneva
    • 1
  • Ivan A. Vorobjev
    • 2
  1. 1.Cellular and Molecular Medicine ProgramBoston Children’s HospitalBostonUSA
  2. 2.A.N. Belozersky Institute of Physico-CheM.V. Lomonosov Moscow State UniversityMoscowRussia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3300-6
  • Online ISBN 978-1-4939-3302-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site