Lipid Signaling Protocols

  • Mark G. Waugh

Part of the Methods in Molecular Biology book series (MIMB, volume 1376)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Timothy A. Couttas, Anthony S. Don
    Pages 23-33
  3. Avanti Gokhale, Pearl V. Ryder, Stephanie A. Zlatic, Victor Faundez
    Pages 35-42
  4. Joachim Füllekrug, Margarete Poppelreuther
    Pages 43-53
  5. Matthew C. Gage, Benoit Pourcet, Inés Pineda-Torra
    Pages 77-85
  6. Massimo Aureli, Sara Grassi, Sandro Sonnino, Alessandro Prinetti
    Pages 107-131
  7. Abasha Lewis, Shang-Yi Tsai, Tsung-Ping Su
    Pages 133-140
  8. Ricarda A. Busse, Andreea Scacioc, Amanda M. Schalk, Roswitha Krick, Michael Thumm, Karin Kühnel
    Pages 155-162
  9. Wonhwa Cho, Hyunjin Kim, Yusi Hu
    Pages 163-174
  10. Matthieu Pierre Platre, Yvon Jaillais
    Pages 175-194
  11. Back Matter
    Pages 255-257

About this book


In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.



cell function ceramides cholesterol-rich lipid rafts detergent-resistant membrane lipid signaling membrane environment phosphoinositides receptor-initiated signaling cascades sphingomyelin

Editors and affiliations

  • Mark G. Waugh
    • 1
  1. 1.University College of London School of Life and Medical SciencesLondonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3169-9
  • Online ISBN 978-1-4939-3170-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site