The Genetic Manipulation of Staphylococci

Methods and Protocols

  • Jeffrey L. Bose

Part of the Methods in Molecular Biology book series (MIMB, volume 1373)

Table of contents

  1. Front Matter
    Pages i-x
  2. Christina N. Krute, Jeffrey L. Bose
    Pages 1-7
  3. Ameya A. Mashruwala, Jeffrey M. Boyd
    Pages 33-41
  4. Melinda R. Grosser, Anthony R. Richardson
    Pages 51-57
  5. Kelsey L. Krausz, Jeffrey L. Bose
    Pages 59-62
  6. Kelsey L. Krausz, Jeffrey L. Bose
    Pages 63-68
  7. Cortney R. Halsey, Paul D. Fey
    Pages 83-87
  8. McKenzie K. Lehman, Jeffrey L. Bose, Kenneth W. Bayles
    Pages 89-96
  9. Vijaya Kumar Yajjala, Todd J. Widhelm, Jennifer L. Endres, Paul D. Fey, Kenneth W. Bayles
    Pages 103-110
  10. Jeffrey L. Bose
    Pages 111-115
  11. Batu K. Sharma-Kuinkel, Thomas H. Rude, Vance G. Fowler Jr.
    Pages 117-130
  12. Ronan K. Carroll, Andy Weiss, Lindsey N. Shaw
    Pages 131-141
  13. Sarah E. Rowe, James P. O’Gara
    Pages 155-167
  14. Back Matter
    Pages 185-186

About this book


This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.


Mutant generation Nucleic acid analysis Pathogenic bacteria Protein function Single nucleotide changes Staphylococcus Whole genome mutant libraries

Editors and affiliations

  • Jeffrey L. Bose
    • 1
  1. 1.University of Kansas Medical CenterKansas CityUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3157-6
  • Online ISBN 978-1-4939-3158-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site